Evidence map›Paper›PMID 40168439›Full record

ArticlePloS one2025

Active surveillance for Theileria orientalis and the invasive Asian longhorned tick (Haemaphysalis longicornis) in three Missouri beef herds.

Rosalie A Ierardi, Savannah M Chance, Celeste Morris, Jacqueline Nunnelley, Solomon O Odemuyiwa, Angela B Royal, Loren Schultz, Zhenyu Shen, Jordyn Young, Ram K Raghavan

Abstract read
In one paragraph

Article in PloS one, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Acute infection with the U.S. isolate ofFrontiers in veterinary science · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rosalie A IerardiVeterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, Missouri, United States of America.ORCID 0000-0002-3330-3759
Savannah M ChanceDivision of Animal Sciences, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, Missouri, United States of America.
Celeste MorrisDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
Jacqueline NunnelleyVeterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, Missouri, United States of America.
Solomon O OdemuyiwaVeterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, Missouri, United States of America.
Angela B RoyalVeterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, Missouri, United States of America.
Loren SchultzDepartment of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
Zhenyu ShenVeterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, Missouri, United States of America.
Jordyn YoungCollege of Veterinary Medicine, University of Missouri, Columbia, Missouri, United States of America.
Ram K RaghavanVeterinary Medical Diagnostic Laboratory, University of Missouri, Columbia, Missouri, United States of America.

Funding

USDA National Institute of Food and Agriculture 7006485
6 · The paper itself

Abstract

Theileria orientalis is a protozoan hemoparasite of cattle vectored by the rapidly emerging invasive Asian longhorned tick (Haemaphysalis longicornis). Theileria-associated bovine anemia (TABA) is easily mistaken for bovine anaplasmosis, which can lead to delayed diagnosis in areas where bovine anaplasmosis is endemic and TABA is newly emerging. Our objective was to surveil for infestation of cattle by H. longicornis and infection with T. orientalis on three Missouri cow-calf operations in counties where H. longicornis is known to be established. A total of 147 apparently healthy adult cows from 3 herds were inspected for ticks. Whole blood was collected for T. orientalis and Anaplasma marginale quantitative PCR and was also used for immediate preparation of blood smears and measurement of packed cell volumes. A total of 527 ticks were collected from the cows and taxonomically identified to the species level. Eighteen H. longicornis, including 9 adult females and 9 nymphs, were collected from 16 cows (Farm A, 2 cows; Farm B, 4 cows; Farm C, 10 cows). Intraerythrocytic T. orientalis organisms were presumptively identified on blood smears from 10 cows. Quantitative PCR screening of blood samples with primers designed to amplify all T. orientalis genotypes detected 11 positive samples (Farm A, 7 cows; Farm B, 3 cows; Farm C, 1 cow). Positive samples were re-tested with probes specific for the Ikeda, Chitose, and Buffeli genotypes, which detected the Chitose genotype in 10 samples and the Ikeda genotype in 1 sample. Detection of T. orientalis with concurrent infestation of cows by H. longicornis within these 3 herds, along with collection of H. longicornis from vegetation on the premises, supports local tick-borne transmission of this emerging pathogen.

Indexed as

Cattle DiseasesIxodidaeTheileriaTheileriasisAnaplasmosisAnimalsCattleFemaleHaemaphysalis longicornisIntroduced SpeciesMissouri

Identifiers

PMID40168439
PMCPMC11960879

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.