Evidence map›Paper›PMID 40167915›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Phosphoproteomics of Cerebrospinal Fluid-Derived Extracellular Vesicles Applied to Primary Central Nervous System Lymphoma.

Yuanyuan Deng, Anton Iliuk, W Andy Tao, Zhuoying Xie

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yuanyuan DengState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China.
Anton IliukDepartment of Biochemistry, Purdue University, West Lafayette, IN, USA.
W Andy TaoDepartment of Biochemistry, Purdue University, West Lafayette, IN, USA.
Zhuoying XieState Key Laboratory of Digital Medical Engineering, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, China. zyxie@seu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebrospinal fluid (CSF)-derived extracellular vesicles (EVs) are significant media for the cell-cell interaction in the central nervous system (CNS). Thus, characterization and analysis of CSF EVs can provide a pile of information for the discovery of molecular biomarkers of CNS diseases. Furthermore, phosphorylation of proteins plays a vital role in the regulation of protein function and cell signal transduction mechanism, and dysfunction of phosphorylation is always associated with the occurrence and progression of disease. Aiming to conduct mass spectrometry (MS)-based phosphoproteomic analysis of CSF EVs, we describe here a complete workflow to isolate EVs from CSF and profile their phosphoproteome, employing EVtrap-based EV isolation, spin-tip PolyMAC-Ti phosphopeptide enrichment and label-free quantitation of phosphoproteome. The approach provides a powerful CSF EV phosphoproteome analysis pipeline that can contribute to the discovery and identification of EV-based molecular markers.

Indexed as

Central Nervous System NeoplasmsExtracellular VesiclesLymphomaPhosphoproteinsProteomeProteomicsBiomarkers, TumorHumansMass SpectrometryPhosphorylationBiomarkers, TumorPhosphoproteinsProteomeCerebrospinal fluidEVtrap PolyMACExtracellular vesiclesLabel-free quantitationPCNSLPhosphoproteomics

Identifiers

PMID40167915

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.