Evidence map›Paper›PMID 40167913›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Analyzing N-Glycans in Cerebrospinal Fluid: A Glycomics Approach.

Cristian D Gutierrez Reyes, Sherifdeen Onigbinde, Joy Solomon, Yehia Mechref

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Cristian D Gutierrez ReyesDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Sherifdeen OnigbindeDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Joy SolomonDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA.
Yehia MechrefDepartment of Chemistry and Biochemistry, Texas Tech University, Lubbock, TX, USA. Yehia.Mechref@ttu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cerebrospinal fluid (CSF) plays a crucial role in direct interaction with the brain, making it an essential sample for identifying molecular markers that emerge as neurological disorders progress. Notably, changes in protein glycosylation are observed in many neurodegenerative diseases. However, analyzing the glycome profile of CSF is challenging due to low protein concentration. N-glycans are chemically modified through permethylation to quantify these changes accurately and analyzed using a highly sensitive liquid chromatography-mass spectrometry (LC-MS) technique. This study outlines a method for preparing samples with optimized conditions for N-glycan digestion combined with a sensitive detection system. The approach has proven reliable and consistent in measuring various permethylated N-glycans extracted from CSF.

Indexed as

GlycomicsPolysaccharidesChromatography, LiquidGlycosylationHumansMass SpectrometryTandem Mass SpectrometryPolysaccharidesCSFLC-MSN-GlycansPermethylation

Identifiers

PMID40167913

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.