Evidence map›Paper›PMID 40167909›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Luminex Multiplex Immunoassay for Proteomic Profiling of Cerebrospinal Fluid.

Joana M Huertas-Pons, Ana Quiroga-Varela

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Joana M Huertas-PonsNeurodegeneration and Neuroinflammation Research Group, Girona Biomedical Research Institute (IDIBGI), Girona, Spain.
Ana Quiroga-VarelaNeurodegeneration and Neuroinflammation Research Group, Girona Biomedical Research Institute (IDIBGI), Girona, Spain. aquiroga@idibgi.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The enzyme-linked immunosorbent assay (ELISA) is commonly used in research and diagnostics but has limitations, including its single-substance detection and large sample volume requirements. In contrast, the Luminex technique, a bead-based sandwich immunoassay combined with flow cytometry, overcomes these limitations by simultaneous measurement of multiple analytes in small volumes. Using dyed micro-beads coated with monoclonal antibodies, this method can detect 100-500 immune markers in cerebrospinal fluid, capturing multiple targets from a single sample. This chapter discusses key pre-analytical factors like sample collection and preservation that influence detection outcomes, highlighting the Luminex technique's adaptability for measuring diverse biomarkers and its advantages in diagnostic applications, reducing variability, and optimizing resources.

Indexed as

ProteomicsBiomarkersEnzyme-Linked Immunosorbent AssayFlow CytometryHumansImmunoassayBiomarkersBead-based immunoassayBiomarker detectionCytokine profilingDiagnostic applicationsMultiplex immunoassay

Identifiers

PMID40167909

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.