Evidence map›Paper›PMID 40167834›Full record

ArticleVeterinary research communications2025

Genetic diversity and population structure of Fasciola gigantica isolated from cattle in Malawi.

Lenson Mogha, Henson Kainga, Nathan Kamanga, Thoko Flav Kapalamula, Catherine Wood, Lian F Thomas, Florence Mutua, Neil Sargison, Kyoko Hayashida, Taiga Tsutsumi and 4 more

Abstract read
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Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Lenson MoghaDepartment of Animal Health and Livestock Development, Chitipa District Council, P.O. Box 1, Chitipa, Malawi.
Henson KaingaDepartment of Veterinary Epidemiology and Public Health, Faculty of Veterinary Medicine, Lilongwe University of Agriculture and Natural Resources, P.O. Box 219, Lilongwe, Malawi.
Nathan KamangaCentral Veterinary Laboratory, P.O. Box 527, Lilongwe, Malawi.
Thoko Flav KapalamulaDepartment of Veterinary Pathobiology, Faculty of Veterinary Medicine, Lilongwe University of Agriculture and Natural Resources, P.O. Box 219, Lilongwe, Malawi.
Catherine WoodDepartment of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Lilongwe University of Agriculture and Natural Resources, P.O. Box 219, Lilongwe, Malawi.
Lian F ThomasNeglected Zoonoses in the One Health Centre in Africa (OHRECA), International Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
Florence MutuaNeglected Zoonoses in the One Health Centre in Africa (OHRECA), International Livestock Research Institute, P.O. Box 30709, Nairobi, 00100, Kenya.
Neil SargisonRoyal (Dick) School of Veterinary Studies and Roslin Institute, Easter Bush Veterinary Centre, University of Edinburgh, Midlothian, EH25 9RG, UK.
Kyoko HayashidaDivision of Education and Collaboration, International Institute for Zoonosis Control, Hokkaido University, Kita 20 Nishi 10, Kitaku, Sapporo, Hokkaido, Japan.
Taiga TsutsumiLaboratory of Parasitology, Graduate School of Infectious Diseases, Hokkaido University, Kita 18 Nishi 9, Kitaku, Sapporo, Hokkaido, Japan.
Naoki HayashiLaboratory of Parasitology, Graduate School of Infectious Diseases, Hokkaido University, Kita 18 Nishi 9, Kitaku, Sapporo, Hokkaido, Japan.
Nariaki NonakaLaboratory of Parasitology, Graduate School of Infectious Diseases, Hokkaido University, Kita 18 Nishi 9, Kitaku, Sapporo, Hokkaido, Japan.
Ryo NakaoLaboratory of Parasitology, Graduate School of Infectious Diseases, Hokkaido University, Kita 18 Nishi 9, Kitaku, Sapporo, Hokkaido, Japan.
Elisha ChatangaDepartment of Veterinary Pathobiology, Faculty of Veterinary Medicine, Lilongwe University of Agriculture and Natural Resources, P.O. Box 219, Lilongwe, Malawi. echatanga@luanar.ac.mw.

Funding

Japan Society for the Promotion of Science KAKENHI JP23K23770 and JP24KK0133
6 · The paper itself

Abstract

Fasciola gigantica is an important trematode that affects the health of animals and humans in tropical and subtropical countries, including Malawi. Information on the genetic diversity and population structure of F. gigantica is important to understanding the parasite`s transmission patterns/ and in monitoring the development of resistance to commonly used anthelmintic agents. This study aimed to analyze the genetic diversity and population structure of Fasciola species collected from cattle at slaughter slabs and abattoirs in selected districts of Malawi. A total of 27 adult liver flukes were collected from cattle at slaughter slabs and abattoirs in the northern region (n = 12), central region (n = 5), and southern region (n = 10). The mitochondrial cytochrome c oxidase I (COI) gene and nicotinamide adenine dinucleotide dehydrogenase 1 (ND1) gene were amplified and the amplicons were sequenced for all samples. The sequences obtained were used to investigate genetic diversity through median-joining networks and phylogenetic analysis. Tajima's D test and Fu's Fs statistics were used to determine the population structure. Based on the analyzed COI and ND1 sequences, all samples were identified as F. gigantica. Single nucleotide polymorphisms (SNPs) were identified at 18 and 17 positions for COI and ND1 genes, resulting in 10 and 5 haplotypes, respectively. The haplotype diversities were 0.867 and 0.479 for COI and ND1 gene sequences, respectively. The population genetic structure indices showed a population that has undergone a recent expansion. This study provides baseline epidemiological data on the genetic diversity and population structure of F. gigantica in Malawi; which is important for its control.

Indexed as

Cattle DiseasesFasciolaFascioliasisGenetic VariationAnimalsCattleElectron Transport Complex IVMalawiPhylogenyPolymorphism, Single NucleotideElectron Transport Complex IVCattleFasciola giganticaGenetic diversityMalawiPopulation structure

Identifiers

PMID40167834

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.