Evidence map›Paper›PMID 40166809›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2025

Functional Microendoscopy Reveals Calcium Responses of Single Cells in Tracheal Tuft Cells and Kidney Podocytes.

Tobias A Dancker, Mohamed Ibrahem Elhawy, Ramona Rittershauß, Qinghai Tian, Yvonne Schwarz, Markus D A Hoffmann, Christopher Carlein, Amanda Wyatt, Vanessa Wahl, Daniel Speyerer and 7 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors.

Tobias A DanckerMolecular Imaging, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.ORCID 0009-0003-1635-361X
Mohamed Ibrahem ElhawyInstitute of Anatomy and Cell Biology, Saarland University, Kirrberger Str. 100, building 61, 66421, Homburg, Saarland, Germany.ORCID 0000-0002-8252-1832
Ramona RittershaußMolecular Cell Biology, Center for Molecular Signaling (PZMS), Saarland University, Kirrberger Str. 100, building 61, 66421, Homburg, Saarland, Germany.
Qinghai TianMolecular Cell Biology, Center for Molecular Signaling (PZMS), Saarland University, Kirrberger Str. 100, building 61, 66421, Homburg, Saarland, Germany.
Yvonne SchwarzMolecular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.
Markus D A HoffmannBiophysics, Center for Human and Molecular Biology (ZHMB), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.
Christopher CarleinBiophysics, Center for Human and Molecular Biology (ZHMB), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.
Amanda WyattExperimental Pharmacology, Center for Molecular Signaling (PZMS), Saarland University, Kirrberger Str. 100, building 45&46, 66421, Homburg, Saarland, Germany.
Vanessa WahlExperimental Pharmacology, Center for Molecular Signaling (PZMS), Saarland University, Kirrberger Str. 100, building 45&46, 66421, Homburg, Saarland, Germany.
Daniel SpeyererInstitute of Anatomy and Cell Biology, Saarland University, Kirrberger Str. 100, building 61, 66421, Homburg, Saarland, Germany.
Alaa KandahMolecular Imaging, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.
Ulrich BoehmExperimental Pharmacology, Center for Molecular Signaling (PZMS), Saarland University, Kirrberger Str. 100, building 45&46, 66421, Homburg, Saarland, Germany.ORCID 0000-0003-2436-6907
Leticia Prates RomaBiophysics, Center for Human and Molecular Biology (ZHMB), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.
Dieter BrunsMolecular Neurophysiology, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.
Peter LippMolecular Cell Biology, Center for Molecular Signaling (PZMS), Saarland University, Kirrberger Str. 100, building 61, 66421, Homburg, Saarland, Germany.ORCID 0000-0003-4728-9174
Gabriela Krasteva-ChristInstitute of Anatomy and Cell Biology, Saarland University, Kirrberger Str. 100, building 61, 66421, Homburg, Saarland, Germany.ORCID 0000-0002-0113-521X
Marcel A LauterbachMolecular Imaging, Center for Integrative Physiology and Molecular Medicine (CIPMM), Saarland University, Kirrberger Str. 100, building 48, 66421, Homburg, Saarland, Germany.ORCID 0009-0008-8145-6873

Funding

Deutsche Forschungsgemeinschaft KR4338/1-2Deutsche Forschungsgemeinschaft TRR 152 project P22Studienstiftung des Deutschen VolkesUniversität des Saarlandes HomFor2023NachwuchsförderungUniversität des Saarlandes HomForexzellent2023Universität des Saarlandes NanoBioMedSeedGrant"InVivoFiber-BasedImaging"
6 · The paper itself

Abstract

Microendoscopy, a crucial technology for minimally invasive investigations of organs, facilitates studies within confined cavities. However, conventional microendoscopy is often limited by probe size and the constraint of using a single excitation wavelength. In response to these constraints, a multichannel microendoscope with a slender profile of only 360 µm is engineered. Functional signals both in situ and in vivo are successfully captured from individual single cells, employing a specially developed software suite for image processing, and exhibiting an effective resolution of 4.6 µm, allowing for the resolution of subcellular neuronal structures. This system enabled the first examination of calcium dynamics in vivo in murine tracheal tuft cells (formerly named brush cells) and in situ in kidney podocytes. Additionally, it recorded ratiometric redox reactions in various biological settings, including intact explanted organs and pancreatic islet cultures. The flexibility and streamlined operation of the microendoscopic technique open new avenues for conducting in vivo research, allowing for studies of tissue and organ function at cellular resolution.

Indexed as

CalciumEndoscopyKidneyPodocytesTracheaAnimalsMiceTuft CellsCalciumfunctional fluorescence microendoscopyGRIN lensimage processingintravital microscopykidney podocytetracheal brush cellstracheal tuft cells

Identifiers

PMID40166809
PMCPMC12105425

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.