Evidence map›Paper›PMID 40166301›Full record

ArticlebioRxiv : the preprint server for biology2025

Human Neonatal MR1T Cells Have Diverse TCR Usage, are Less Cytotoxic and are Unable to Respond to Many Common Childhood Pathogens.

Dylan Kain, Wael Awad, G W McElfresh, Meghan Cansler, Gwendolyn M Swarbrick, Kean Chan Yew Poa, Conor McNeice, Gregory Boggy, Katherine Rott, Megan D Null and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Dylan KainDivision of Infectious Diseases, Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA.ORCID 0000-0001-5890-2742
Wael AwadInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
G W McElfreshOregon National Primate Research Center, OHSU, Beaverton, OR, USA.
Meghan CanslerDivision of Infectious Diseases, Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA.
Gwendolyn M SwarbrickDivision of Infectious Diseases, Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA.
Kean Chan Yew PoaInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Conor McNeiceInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Gregory BoggyOregon National Primate Research Center, OHSU, Beaverton, OR, USA.
Katherine RottDivision of Infectious Diseases, Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA.
Megan D NullDivision of Infectious Diseases, Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA.
David M LewinsohnDivision of Pulmonary, Allergy and Critical Care Medicine, Department of Medicine, Oregon Health & Science University, Portland, OR, USA.
Jamie RossjohnInfection and Immunity Program and Department of Biochemistry and Molecular Biology, Biomedicine Discovery Institute, Monash University, Clayton, Victoria, Australia.
Benjamin N BimberOregon National Primate Research Center, OHSU, Beaverton, OR, USA.ORCID 0000-0001-8092-3080
Deborah A LewinsohnDivision of Infectious Diseases, Department of Pediatrics, Oregon Health & Science University, Portland, OR, USA.

Funding

Lung Resident, MR1-Restricted T Cells: Association with Differential Outcomes Following Exposure to M. TuberculosisR01AI134790 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI LEWINSOHN, DAVID M., WALZL, GERHARD · 2018 to 2022
$3.3M
Investigating the basis of MAIT cell antigen potencyR01AI148407 · NIAID · UNIVERSITY OF MELBOURNE · PI MCCLUSKEY, JAMES · 2020 to 2024
$2.7M
Memory, Phenotype, and Function of TB-reactive Human MR1 Restricted T cellsR01AI129980 · NIAID · OREGON HEALTH & SCIENCE UNIVERSITY · PI LEWINSOHN, DAVID M., LEWINSOHN, DEBORAH A. · 2019 to 2022
$2.4M
BLRD VA I01 BX000533NIAID NIH HHS R01 AI129980NIAID NIH HHS R01 AI134790NIAID NIH HHS R01 AI148407
6 · The paper itself

Abstract

Neonatal sepsis is a leading cause of childhood mortality. Understanding immune cell development can inform strategies to combat this. MR1-restricted T (MR1T) cells can be defined by their recognition of small molecules derived from microbes, self, and drug and drug-like molecules, presented by the MHC class 1-related molecule (MR1). In healthy adults, the majority of MR1T cells express an invariant α-chain; TRAV1-2/TRAJ33/12/20 and are referred to as mucosal-associated invariant T (MAIT) cells. Neonatal MR1T cells isolated from cord blood (CB) demonstrate more diversity in MR1T TCR usage, with the majority of MR1-5-OP-RU-tetramer(+) cells being TRAV1-2(-). To better understand this diversity, we performed single-cell-RNA-seq/TCR-seq (scRNA-seq/scTCR-seq) on MR1-5-OP-RU-tetramer(+) cells from CB (n=5) and adult participants (n=5). CB-derived MR1T cells demonstrate a less cytotoxic/pro-inflammatory phenotype, and a more diverse TCR repertoire. A panel of CB and adult MAIT and TRAV1-2(-) MR1T cell clones were generated, and CB-derived clones were unable to recognize several common riboflavin-producing childhood pathogens (

Indexed as

MAIT cellsMR1MR1T cellsNeonatal sepsisSingle-cell sequencingT cell developmentTuberculosis

Identifiers

PMID40166301
PMCPMC11956999

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.