ArticlebioRxiv : the preprint server for biology2025
Prior epigenetic status predicts future susceptibility to seizures in mice.
Benjamin D Boros, Mariam A Gachechiladze, Juanru Guo, Dylan A Galloway, Shayna M Mueller, Mark Shabsovich, Allen Yen, Alexander J Cammack, Tao Shen, Robi D Mitra and 2 more
Abstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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4 · The recordCorrections and comments
5 · Who and what moneyAuthors and funding
12 authors.
Benjamin D BorosDepartment of Neurology, Washington University School of Medicine, St. Louis, MO 63110.ORCID 0000-0003-2708-4112 Mariam A GachechiladzeDepartment of Genetics, Washington University School of Medicine, St. Louis, MO 63110.ORCID 0000-0003-0004-425X Juanru GuoDepartment of Genetics, Washington University School of Medicine, St. Louis, MO 63110.
Dylan A GallowayDepartment of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Shayna M MuellerDepartment of Genetics, Washington University School of Medicine, St. Louis, MO 63110.
Mark ShabsovichDepartment of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Allen YenDepartment of Genetics, Washington University School of Medicine, St. Louis, MO 63110.
Alexander J CammackDepartment of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Tao ShenDepartment of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Joseph D DoughertyDepartment of Genetics, Washington University School of Medicine, St. Louis, MO 63110.ORCID 0000-0002-6385-3997 Timothy M MillerDepartment of Neurology, Washington University School of Medicine, St. Louis, MO 63110.
Funding
Molecular recording to predict cell fate decisions and animal behaviorRF1MH126723 · NIMH · WASHINGTON UNIVERSITY · PI DOUGHERTY, JOSEPH D, MITRA, ROBI D · 2021 to 2021
$3.7MPARALLEL ANALYSIS OF TRANSCRIPTION AND PROTEIN-DNAINTERACTIONS IN SINGLE CNS CELLSRF1MH117070 · NIMH · WASHINGTON UNIVERSITY · PI DOUGHERTY, JOSEPH D, MITRA, ROBI D · 2018 to 2020
$3.2MDeciphering epigenetically-regulated pathways to improve targeted therapy for invasion and metastasis in head and neck cancerR01DE032865 · NIDCR · WASHINGTON UNIVERSITY · PI Robi D Mitra, Sidharth Venkata Puram · 2023 to 2026
$2.4MBoosting protein synthesis using antisense oligonucleotides to treat neurodegenerationF30AG082394 · NIA · WASHINGTON UNIVERSITY · PI Ben David Boros · 2024 to 2026
$125kAntisense oligonucleotide-based upregulation of neurodevelopmental disorder gene MYT1LF30MH136688 · NIMH · WASHINGTON UNIVERSITY · PI GACHECHILADZE, MARIAM ALEXANDRA · 2024 to 2025
$72kNIA NIH HHS F30 AG082394NIDCR NIH HHS R01 DE032865NIMH NIH HHS F30 MH136688NIMH NIH HHS RF1 MH117070NIMH NIH HHS RF1 MH126723
6 · The paper itselfAbstract
Wide variation of responses to identical stimuli presented to genetically inbred mice suggests the hypothesis that stochastic epigenetic variation during neurodevelopment can mediate such phenotypic differences. However, this hypothesis is largely untested since capturing pre-existing molecular states requires non-destructive, longitudinal recording. Therefore, we tested the potential of Calling Cards (CC) to record transient neuronal enhancer activity during postnatal development, and thereby associate epigenetic variation with a subsequent phenotypic presentation - degree of seizure response to the pro-convulsant pentylenetetrazol. We show that recorded differences in epigenetics at 243 loci predict a severe vs. mild response, and that these are enriched near genes associated with human epilepsy. We also validated pharmacologically a seizure-modifying role for two novel genes,
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PMID40166300
PMCPMC11957114
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