Evidence map›Paper›PMID 40166251›Full record

ArticlebioRxiv : the preprint server for biology2025

NitrOFF: An engineered fluorescent biosensor to illuminate nitrate transport in living cells.

Mariah A Cook, Jonathan D Smailys, Ke Ji, Shelby M Phelps, Jasmine N Tutol, Wantae Kim, Whitney S Y Ong, Weicheng Peng, Caden Maydew, Y Jessie Zhang and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Mariah A CookDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Jonathan D SmailysDepartment of Molecular Biosciences, University of Texas, Austin, TX 78712.
Ke JiDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Shelby M PhelpsDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Jasmine N TutolDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Wantae KimMcKetta Department of Chemical Engineering, University of Texas, Austin, TX 78712.
Whitney S Y OngDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Weicheng PengDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Caden MaydewDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.
Y Jessie ZhangDepartment of Molecular Biosciences, University of Texas, Austin, TX 78712.
Sheel C DodaniDepartment of Chemistry and Biochemistry, The University of Texas at Dallas, Richardson, TX 75080.

Funding

Optical Imaging Tools for Elucidating the Roles of Anions and Anionic Modifications in Cellular SignalingR35GM128923 · NIGMS · UNIVERSITY OF TEXAS DALLAS · PI Sheel Dodani · 2018 to 2026
$3.4M
Deciphering the phosphorylation pattern of RNA polymerase II for eukaryotic transcriptionR35GM148356 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Yan Jessie Zhang · 2023 to 2026
$2.3M
NIGMS NIH HHS R35 GM128923NIGMS NIH HHS R35 GM148356
6 · The paper itself

Abstract

The duality of nitrate is nowhere best exemplified than in human physiology - a detrimental pollutant but also a protective nutrient and signaling ion - particularly as connected to reactive nitrogen oxides. Aside from limited insights into nitrate uptake and storage, foundational nitrate biology has lagged. Genetically encoded fluorescent biosensors can address this gap with real-time imaging. However, imaging technologies for mammalian cell applications remain rare. Here, we set out to design and engineer a two-domain chimera fusing the split green fluorescent protein EGFP and the nitrate recognition domain NreA from

Indexed as

biosensordirected evolutiongreen fluorescent proteinnitrateX-ray crystallography

Identifiers

PMID40166251
PMCPMC11957115

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.