ArticleMicroscopy research and technique2025
Alternatives for Uranyl Acetate Negative Staining With Respect to the Resolution Obtained After Single Particle Analysis of Erythrocruorin.
Article in Microscopy research and technique, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
4 citing papers in PubMed.
- Multiscale Analysis of the Impact of the Isomerization of 4-Amine Tetraortho-Azobenzenes on the Structure and Dynamics of Tubular Micelles.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Different approaches to assess extracellular vesicles from bovine colostrum.JDS communications · 2026Article
- Synthesis and uranyl(VI) extraction performance of a calix[4]pyrrole-tetrahydroxamic acid receptor.Beilstein journal of organic chemistry · 2026Article
- Alternatives for Uranyl Acetate Negative Staining With Respect to the Resolution Obtained After Single Particle Analysis of Erythrocruorin.Microscopy research and technique · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although cryo-electron microscopy is widely used for determining high-resolution structures, negative staining of proteins and supramolecular aggregates remain a valuable screening method, especially for low-abundant proteins. The most commonly used contrasting agent is uranyl acetate (UAc). However, due to its toxicity and the strong regulations connected to the use of uranium compounds, there is an ongoing search for alternative stains, with the focus being shifted to lanthanide and tungsten compounds. In this study, the performance of neodymium acetate (NdAc), europium acetate (EuAc), ytterbium acetate (YbAc), phosphotungstic acid, and the commercially available UA-Zero as negative staining agents for single molecule electron microscopy is evaluated. We focus not only on the observed contrast but also on the resolution that can be obtained after negative staining and single-particle analysis. The findings demonstrate that NdAc, EuAc, and UA-Zero can be good alternatives for the overall superior UAc. Additionally, we explore the working pH range of the stains and discuss the usability of the compounds in terms of availability and regulations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.