Evidence map›Paper›PMID 40163310›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

High Efficiency Lentiviral Transduction of Colon Organoids Using Reversible 2D/3D Culture Techniques.

Florian Rathje, Martina M Sykora, Fritz Aberger, Peter W Krenn

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Florian RathjeDepartment of Biosciences and Medical Biology, Center for Tumor Biology and Immunology (CTBI), Cancer Cluster Salzburg, Paris Lodron University of Salzburg, Salzburg, Austria.
Martina M SykoraDepartment of Biosciences and Medical Biology, Center for Tumor Biology and Immunology (CTBI), Cancer Cluster Salzburg, Paris Lodron University of Salzburg, Salzburg, Austria.
Fritz AbergerDepartment of Biosciences and Medical Biology, Center for Tumor Biology and Immunology (CTBI), Cancer Cluster Salzburg, Paris Lodron University of Salzburg, Salzburg, Austria.
Peter W KrennDepartment of Biosciences and Medical Biology, Center for Tumor Biology and Immunology (CTBI), Cancer Cluster Salzburg, Paris Lodron University of Salzburg, Salzburg, Austria. peter.krenn@plus.ac.at.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Organoids are a promising research tool for studying tissue development and disease in vitro. While organoids are frequently considered a replacement or complementary model for in vivo mouse experiments, exploiting their full potential often requires genetically engineered mice as a source of transgenic stem cells, also because genetic manipulation of organoids is rather inefficient and cumbersome. Here, we describe an alternative and optimized murine colon organoid manipulation protocol that reversibly and temporarily interrupts the 3D organoid structure for short-term 2D monolayer culture. This approach allows highly efficient viral transduction and genetic manipulation of stem cells in a 2D setting, followed by 3D stem cell embedding and restoration of the original organoid architecture. This method greatly improves the efficiency of lentiviral-mediated genetic manipulation of organoids and increases their potential applications in CRISPR/Cas9 and compound screens, immune-competent co-cultures, and disease modeling.

Indexed as

Cell Culture TechniquesColonLentivirusOrganoidsTransduction, GeneticAnimalsCRISPR-Cas SystemsGenetic VectorsHumansMiceStem Cells2D/3D organoid cultureColon organoidsGenetic manipulationStem cellsViral transduction

Identifiers

PMID40163310

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.