ArticleInvestigative ophthalmology & visual science2025
Vitreous Fibrillar Structure and Interfibrillar Composition in Adult Mice.
Article in Investigative ophthalmology & visual science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Principles of resident tissue macrophages revealed by the eye.Advances in immunology · 2026Review
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Purpose: Although mice are widely used models for human eye diseases, knowledge of their vitreous is scarce and fragmentary. This study characterizes the structure and composition of vitreous fibrils and their interfibrillar space. Given the role of this fibrillar network in maintaining the vitreous' biomechanical properties, the impact of intravitreal injections on vitreous architecture was also explored. Methods: Healthy adult C57/BL6J mice were studied. Classical histological techniques, picrosirius red-polarization, immunofluorescence, lectin histochemistry, and hyaluronic acid binding protein assays were used to examine vitreous fibrils and interfibrillar space. For the analysis of distribution of components along the fibrils, the Airyscan microscopy was used to achieve higher resolution. Results: Vitreous fibrils consist of fibrillar collagen, glycoproteins (fibrillin 1, fibronectin, laminin, and collagen IV), N-acetyl galactosamine, and hyaluronan, all absent in the interfibrillar space. A single sterile intravitreal saline injection induced an inflammatory insult, characterized by increased fibril density and macrophage/hyalocyte invasion. Lysosome-associated membrane protein 1 (Lamp1) immunohistochemistry suggests these cells may remove fibrils via phagocytosis and activate a remodeling process in the vitreous. Conclusions: This study enhances understanding of the mouse vitreous structure, suggesting fibrils are composed of glycoprotein-wrapped collagen cores. Furthermore, the absence of hyaluronan and glycoproteins between fibrils may explain lower viscosity in mice compared with humans. Intravitreal injections as an inflammatory insult disrupt fibril networks and activate macrophages/hyalocytes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.