Evidence map›Paper›PMID 40162804›Full record

ArticleJournal of clinical microbiology2025

Improvement of the diagnosis of intestinal protozoa using a multiplex qPCR strategy compared to classical microscopy: a prospective study on 3,500 stool samples over 3 years.

Florence Robert-Gangneux, Xavier Duval, Clément Cazala, Sorya Belaz, Anne Dupuis, Hélène Guegan, Brice Autier, Jean-Pierre Gangneux

Abstract readComparative Study
In one paragraph

Article in Journal of clinical microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Comparative evaluation of Midi ParasepParasites & vectors · 2026
    Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Florence Robert-GangneuxLaboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.ORCID 0000-0003-4493-596X
Xavier Duval *Laboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.
Clément Cazala *Laboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.
Sorya BelazLaboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.
Anne DupuisLaboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.
Hélène GueganLaboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.ORCID 0000-0001-6338-3094
Brice AutierLaboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.ORCID 0000-0001-6945-4359
Jean-Pierre GangneuxLaboratoire de parasitologie et mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Commercial multiplex real-time PCR (qPCR assays) are now widely used for the diagnosis of intestinal protozoan infections, but few prospective studies evaluated their performances on large patient cohorts. We extracted data from our information system from 1 January 2021 to 15 March 2024 and included all stool samples analyzed in routine. Parasites were searched using a multiplex PCR (AllPlex Gastrointestinal Panel assay, Seegene) and microscopic examination with two concentration methods. Acid-fast staining was performed when

Indexed as

FecesIntestinal Diseases, ParasiticMicroscopyMultiplex Polymerase Chain ReactionProtozoan InfectionsReal-Time Polymerase Chain ReactionAdolescentAdultAgedAged, 80 and overBlastocystisChildChild, PreschoolCryptosporidiumDientamoebaEntamoeba histolyticadiagnosticsgastrointestinal infectionintestinal parasitesmolecular methodsstool

Identifiers

PMID40162804
PMCPMC12077082

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.