Evidence map›Paper›PMID 40162755›Full record

ArticleMicrobiology spectrum2025

Identification of essential genes by transposon insertion sequencing and genome-scale metabolic model construction in

Yongqing Zhang, Ruotong Gong, Menglei Liang, Liangsheng Zhang, Xiujian Liu, Jingzi Zeng, Mengli Yan, Dexin Qiu, Rui Zhou, Qi Huang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Decoding gene essentiality inMicrobiology spectrum · 2025
    Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Yongqing ZhangNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.ORCID 0009-0005-2304-0982
Ruotong GongNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.
Menglei LiangNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.
Liangsheng ZhangNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.
Xiujian LiuNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.
Jingzi ZengNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.
Mengli YanNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.
Dexin QiuCollege of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
Rui ZhouNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.ORCID 0000-0002-7903-9965
Qi HuangNational Key Laboratory of Agricultural Microbiology, Huazhong Agriculture University, Wuhan, Hubei, China.ORCID 0000-0002-1600-1169

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bacterial essential genes are indispensable for the survival of bacteria and therefore are attractive targets for novel anti-microbial drugs. Identifying essential genes provides a roadmap for developing novel antibiotics and anti-microbial therapies. In this study, combining high-throughput transposon sequencing (Tn-seq) and genome-scale metabolic model (GEM) construction, essential genes of IMPORTANCE: Anti-microbial resistance (AMR) presents an escalating challenge, making anti-microbial drug development an urgent need. Bacterial essential genes represent promising targets for anti-microbial drugs. However, conventional approaches to identifying bacterial essential genes are time and labor intensive. Techniques such as Tn-seq and GEM construction offer a high-throughput approach for this identification.

Indexed as

DNA Transposable ElementsGenes, EssentialGenome, BacterialStreptococcus suisAnti-Bacterial AgentsHigh-Throughput Nucleotide SequencingMetabolic Networks and PathwaysMutagenesis, InsertionalAnti-Bacterial AgentsDNA Transposable Elementsantimicrobial drug targetsessential genesgenome-scale metabolic modeltransposon sequencing

Identifiers

PMID40162755
PMCPMC12053914

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.