Evidence map›Paper›PMID 40161946›Full record

ArticleBioImpacts : BI2025

A procedure for DNA methylation assessment in osteoporosis-related gene promoters of umbilical cord blood: A study on the Prospective Epidemiological Research Studies in Iran (PERSIAN) birth cohort.

Sadegh Baradaran Mahdavi, Seyed Morteza Javadirad, Mahsa Rafieian, Parnian Poursafa, Vajihe Azimian Zavareh, Seyede Shahrbanoo Daniali, Motahar Heidari-Beni, Masoomeh Goodarzi-Khoigani, Babak Vahdatpour, Hossein Mirhendi and 1 more

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Article in BioImpacts : BI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Sadegh Baradaran MahdaviDepartment of Physical Medicine and Rehabilitation, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0002-1956-1372
Seyed Morteza JavadiradDepartment of Cell and Molecular Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran.ORCID https://orcid.org/0000-0002-2891-2293
Mahsa RafieianChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0003-2871-0453
Parnian PoursafaChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0001-7769-992X
Vajihe Azimian ZavarehCore Research Facilities (CRF), Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0001-6235-9180
Seyede Shahrbanoo DanialiChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0003-3783-778X
Motahar Heidari-BeniChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0001-5543-5536
Masoomeh Goodarzi-KhoiganiChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0003-3799-2333
Babak VahdatpourDepartment of Physical Medicine and Rehabilitation, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0002-3325-0538
Hossein MirhendiCore Research Facilities (CRF), Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0002-4651-5224
Roya KelishadiChild Growth and Development Research Center, Research Institute for Primordial Prevention of Non-Communicable Disease, Isfahan University of Medical Sciences, Isfahan, Iran.ORCID https://orcid.org/0000-0001-7455-1495

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: It is believed that DNA methylation can modify disease susceptibility in response to environmental factors as early as the perinatal period. In this study, we aimed to present a streamlined DNA methylation analysis procedure for osteoporosis-related gene promoters in the umbilical cord blood. Methods: The Prospective Epidemiological Research Studies in Iran (PERSIAN) birth cohort was established in 2016. In this study, a total of 300 umbilical cord blood samples were collected at the time of delivery. For all samples, DNA was extracted and converted using sodium bisulfite. Multiple primer sets were designed for Results: For Conclusion: BSP can be efficiently used to investigate the methylation of target gene promoters in umbilical cord blood DNA.

Indexed as

Birth cohortEpigenomicsNA methylationNon-communicable diseasesPrenatal exposure delayed effectsPromoter regions

Identifiers

PMID40161946
PMCPMC11954747

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