Evidence map›Paper›PMID 40160213›Full record

ArticleIn vitro models2025

A comparative analysis of vitrification and two slow freezing methods for gonocyte-containing neonatal calf testicular tissue and subsequent in vitro culture.

Shiyan Tang, Celine Jones, Jill Davies, Sheila Lane, Kevin Coward

Abstract read
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In one paragraph

Article in In vitro models, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Shiyan TangNuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU UK.
Celine JonesNuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU UK.
Jill DaviesOxford Cell & Tissue Biobank, Children's Hospital Oxford, Oxford University Hospitals NHS Foundation Trust, Oxford, OX3 9DU UK.
Sheila LaneDepartment of Paediatric Oncology and Haematology, Children's Hospital Oxford, Oxford University Hospitals NHS Foundation Trust, Oxford, OX3 9DU UK.
Kevin CowardNuffield Department of Women's and Reproductive Health, University of Oxford, Women's Centre, John Radcliffe Hospital, Oxford, OX3 9DU UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The cryopreservation of neonatal testicular tissue containing gonocytes is crucial for preserving genetic diversity, advancing research, and developing reproductive technologies. In this study, we investigated three cryopreservation techniques, slow freezing (in which the rate of freezing was controlled or uncontrolled) and vitrification, using neonatal bovine testicular tissues containing gonocytes, followed by in vitro culture to evaluate cell functionality. Vitrification resulted in a significantly lower proportion (19.15 ± 1.82%) of seminiferous tubules with > 70% attachment to the basement membrane in comparison to both the controlled slow freezing group (47.89 ± 10.98%) and the uncontrolled slow freezing group (39.05 ± 4.15%) (

Indexed as

Fertility preservationGerm cellsImmature testicular tissueIn vitro cultureSlow freezingSpermatogonial stem cellsVitrification

Identifiers

PMID40160213
PMCPMC11950551

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.