ArticleIn vitro models2025
A standardized in vitro bioengineered skin for penetrating wound modeling.
Article in In vitro models, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed.
- From 1D microbiological assays to 3D advanced skin models: enhancing preclinical strategies to unravel the impact of bioactive textiles on the human skin microbiome.Frontiers in cellular and infection microbiology · 2025Review
- Engineering a 3D wounded skin equivalent to study early inflammatory and regenerative responsesFrontiers in bioengineering and biotechnology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the years, significant progress has been made in developing cost-effective and ethical in vitro bioengineered skin substitutes to study cutaneous wound healing processes. Rodents and small animal models are not optimal due to physiological differences in their skin compared to human skin. The generation of reproducible and precise wounds is key to obtaining comparable results. We created a three-dimensional skin wounding model by growing a fully differentiated, stratified squamous epithelium from human keratinocytes at an air-liquid interface on a type I collagen scaffold concealed with human dermal BJ fibroblasts. To generate the wounds, a stamp with incorporated needles with a length of 250 µm was used to puncture the epidermis to produce standardized wounds. The stamping needle technique is a practical and inexpensive method for creating length-tailored wounds on three-dimensional skin models. The effectiveness of this technique in treating 3D skin models was demonstrated, accompanied by an evaluation of the model's functionality in terms of cell proliferation, differentiation, and immunological characteristics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.