Evidence map›Paper›PMID 40158017›Full record

ArticleScientific reports2025

Endometrial injection of embryo culture supernatant adversely affects assisted reproductive technology outcomes in frozen thawed embryo transfer cycles.

Ayumu Ito, Yukiko Katagiri, Kentaro Nakaoka, Mami Unagami, Koichiro Ichizawa, Kenji Amano, Yukiko Tokuda, Satoko Oigawa, Masato Yoneyama, Takahiro Tsuchiya and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Ayumu ItoDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan. ayumu.itou@med.toho-u.ac.jp.
Yukiko KatagiriDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Kentaro NakaokaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Mami UnagamiReproduction Center, Toho University Omori Medical Center, Tokyo, Japan.
Koichiro IchizawaReproduction Center, Toho University Omori Medical Center, Tokyo, Japan.
Kenji AmanoReproduction Center, Toho University Omori Medical Center, Tokyo, Japan.
Yukiko TokudaReproduction Center, Toho University Omori Medical Center, Tokyo, Japan.
Satoko OigawaReproduction Center, Toho University Omori Medical Center, Tokyo, Japan.
Masato YoneyamaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Takahiro TsuchiyaDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Mami SekiguchiDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Mayuko FuruiDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Nahomi UmemuraDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Yuko TamakiDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.
Koichi NagaoReproduction Center, Toho University Omori Medical Center, Tokyo, Japan.
Masahiko NakataDepartment of Obstetrics and Gynecology, Faculty of Medicine, Toho University, Tokyo, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We assessed the effects of endometrial injection of embryo culture supernatants on assisted reproductive technology (ART) outcomes in frozen-thawed embryo transfer (FET) cycles. Hormone replacement therapy cycles with blastocyst transfer on day 5 after fertilization were included, excluding cycles potentially affecting implantation. The stimulation of endometrial embryo transfer (SEET) group (118 cycles) received endometrial injections of the embryo culture supernatant, while the blastocyst transfer (BT) group (878 cycles) did not. Maternal age was significantly higher in the SEET than in the BT group (p < 0.05). The implantation, ongoing pregnancy, and live birth rates were significantly lower (all p < 0.05) in the SEET than in the BT group. Clinical pregnancy and miscarriage rates were not significantly different between the groups. Age-adjusted odds ratios (ORs) (95% confidence interval [CI]) for implantation and miscarriage were 1.02(0.69-1.52) and 1.97 (1.04-3.72), respectively, in the SEET group. Multivariate analysis was performed, including female age, BMI, AMH, and other potential factors influencing ART outcomes. The adjusted odds ratios (95% CI) for clinical pregnancy and miscarriage in the SEET group were 1.00 (0.63-1.58) and 2.08 (1.02-4.24), respectively. Additionally, the adjusted odds ratio for miscarriage was 0.732 (0.22-2.47) in patients aged < 37 years and 4.63 (1.61-13.3) in those aged ≥ 37 years. In conclusion, endometrial injection of the embryo culture supernatant may adversely affect ART outcomes in FET cycles, especially in maternal age ≥ 37 years.

Indexed as

Embryo Culture TechniquesEmbryo TransferEndometriumReproductive Techniques, AssistedAbortion, SpontaneousAdultCryopreservationEmbryo ImplantationFemaleFertilization in VitroHumansMaternal AgePregnancyPregnancy OutcomePregnancy RateAssisted reproductive technologyEmbryo culture supernatantEndometrial injectionFrozen-thawed embryo transferRepeated implantation failureStimulation of endometrium embryo transfer

Identifiers

PMID40158017
PMCPMC11954985

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.