ArticleJournal of the American Chemical Society2025
Exquisite Complex Reaction Cascade in the Natural 1,2,4-Triazine Assembly.
Article in Journal of the American Chemical Society, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Sustainable Biocatalytic Cascades: A Versatile Tool in the Synthesis of Natural Products With Therapeutic Potential.Chembiochem : a European journal of chemical biology · 2026Review
- Microbial unusual gene clusters without prominent core enzymes: natural products, biosynthesis and genome mining.The Journal of antibiotics · 2026Review
- A bibliometric review of triazine hybrids: synthesis, reactions, and applications spanning the last quarter-century (2000-2025).RSC advances · 2025Review
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Authors and funding
3 authors.
Funding
Abstract
1,2,4-Triazine ring is a scaffold widely found in biologically active compounds, but how nature makes it remains enigmatic. In this study, we unveil the complex enzymatic and nonenzymatic cascade reactions that assemble the 1,2,4-triazine moiety found in the structures of the natural products pseudoiodinine and toxoflavin. Through biochemical studies, isotope labeling, and the application of substrate analogues, we propose a plausible pathway for the 1,2,4-triazine assembly from a common precursor in riboflavin biosynthesis. This process involves four two-electron oxidation steps, C-N bond formation, decarboxylation, and the N-N bond forming step catalyzed by a metal-dependent WD40-repeat (WDR) protein. This study thus not only provides the first biocatalytic route for the 1,2,4-triazine assembly but also identifies a previously unrecognized catalytic role of a large WDR protein family.
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Registered trials
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