Evidence map›Paper›PMID 40156747›Full record

ArticleBulletin of experimental biology and medicine2025

Selection of Optimal Protocol for Decellularization of Extracellular Matrix of Mesenchymal Stromal Cells of Human Umbilical Cord Tissue.

E S Kochetova, D K Matveeva, A E Melik-Pashayev, E R Andreeva, Yu A Romanov, L B Buravkova

Abstract read
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Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

E S KochetovaInstitute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
D K MatveevaInstitute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
A E Melik-PashayevInstitute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.
E R AndreevaInstitute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia. andreeva1564@gmail.com.
Yu A RomanovNational Medical Research Center of Cardiology named after Academician E. I. Chazov, Moscow, Russia.
L B BuravkovaInstitute of Biomedical Problems of the Russian Academy of Sciences, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

To obtain extracellular matrix (ECM) of umbilical cord multipotent mesenchymal stromal cells (UC-MSCs), six decellularization protocols were used, based on physical (freeze/thaw) and chemical (Triton-X100, CHAPS, SDS, and NP-40) methods. The quality of decellularization was assessed using phase-contrast and fluorescence microscopy, quantitative image analysis was performed as well. It was shown that protocols based on CHAPS/NH4OH and Triton X-100/NH4OH are optimal for decellularizing UC-MSC layers and provide high-quality ECM. The SDS-based protocol may be useful when certain requirements to matrix structure are specified. The presented data will be in demand for the development of biomedical products.

Indexed as

Decellularized Extracellular MatrixExtracellular MatrixMesenchymal Stem CellsUmbilical CordCells, CulturedHumansOctoxynolSodium Dodecyl SulfateTissue EngineeringDecellularized Extracellular MatrixOctoxynolSodium Dodecyl Sulfatedecellularizationextracellular matrix (ECM)image analysismicroscopymultipotent mesenchymal stromal cells (MSCs) from umbilical cord

Identifiers

PMID40156747

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.