Evidence map›Paper›PMID 40156746›Full record

ArticleBulletin of experimental biology and medicine2025

Nephroblastoma Overexpressed Protein (NOV/CCN3) Elevated Expression of Inflammation Regulators in a Model of Sepsis-Induced Lung Injury.

H Zhu, L Liu, M Yang, X Zhu, J Cai, H Huang

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Article in Bulletin of experimental biology and medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

H ZhuDepartment of Intensive Care Unit, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
L LiuDepartment of Respiratory and Critical Care Medicine, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
M YangDepartment of Intensive Care Unit, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
X ZhuDepartment of Intensive Care Unit, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
J CaiDepartment of Intensive Care Unit, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China.
H HuangDepartment of Intensive Care Unit, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, China. huanghuiya1248@126.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nephroblastoma overexpressed protein (NOV, also named CCN3), a member of the CCN (Cy61, CTGF, and NOV) family, is a critical biological marker of the severity of acute respiratory distress syndrome (ARDS). However, no evidence has been presented that CCN3 directly affects acute lung injury (ALI) or ARDS. Intratracheal infusion of LPS is an established method to simulate sepsis and induce ALI. To examine the effect of CCN3 on ALI, we developed in vivo and in vitro models of this disease on mice and type II alveolar epithelial A549 cells, respectively. To further clarify the role of CCN3 in ALI, we constructed a CCN3 overexpression model based on plasmid transfection. The results showed that CCN3 expression was up-regulated in LPS-induced ALI both in vivo and in vitro; this effect was time- and dose-dependent. ELISA revealed that overexpression of CCN3 increased the levels of proinflammatory cytokines IL-1β and TNFα. Flow cytometry and Western blotting showed that overexpression of CCN3 increased the expression of proapoptotic protein Bax and decreased the expression of anti-apoptotic protein Bcl-2, thereby promoting apoptosis of A549 cells. The results suggest that CCN3 antagonists can inhibit progression of inflammation and the development of apoptosis in lung epithelial cells, thereby exerting a possible therapeutic effect in ALI.

Indexed as

Acute Lung InjuryNephroblastoma Overexpressed ProteinSepsisA549 CellsAnimalsApoptosisbcl-2-Associated X ProteinDisease Models, AnimalHumansInterleukin-1betaLipopolysaccharidesMaleMiceMice, Inbred C57BLProto-Oncogene Proteins c-bcl-2Tumor Necrosis Factor-alphabcl-2-Associated X ProteinCcn3 protein, mouseInterleukin-1betaLipopolysaccharidesNephroblastoma Overexpressed ProteinProto-Oncogene Proteins c-bcl-2Tumor Necrosis Factor-alphaacute lung injuryBaxBcl-2nephroblastoma overexpressed proteinsepsis

Identifiers

PMID40156746

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.