Evidence map›Paper›PMID 40156193›Full record

ArticlePlant communications2025

Divergent fatty acid desaturase 2 is essential for falcarindiol biosynthesis in carrot.

JiaoJiao Zhu, Naveed Ahmad, Xuan Zhou, Wanying He, Jianhua Wang, Changheng Shan, Zelong Chen, Wenjuan Ji, Zhenhua Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

JiaoJiao ZhuJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Naveed AhmadJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Xuan ZhouJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Wanying HeJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Jianhua WangJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Changheng ShanJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zelong ChenJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Wenjuan JiJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
Zhenhua LiuJoint Center for Single Cell Biology, Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: zhenhua.liu@sjtu.edu.cn.

Funding

Non-US Government Research Support type
6 · The paper itself

Abstract

Carrots produce diverse falcarin-type polyacetylenes (PAs), which function as important antipathogenic phytoalexins and have potential as anticancer agents. Despite their abundance in carrot root tissues, the biosynthesis and evolutionary origins of falcarindiol, a C17-PA, remain unknown. Fatty acid desaturase 2 (FAD2) enzymes diversify PAs by introducing various double and/or triple carbon-carbon bonds into fatty acid chains. Here, we use association analysis to identify candidate FAD2 genes involved in falcarindiol biosynthesis. Using a rapid tobacco transient expression system, we found that DcFAD2 enzymes are highly functionally redundant and promiscuous. Combinatorial assays also revealed unexpected synergistic and redirective effects among FAD2 enzymes, further complicating the biosynthetic pathway. CRISPR-Cas9-mediated mutagenesis and overexpression studies identified previously overlooked DcFAD2 hub genes as essential for falcarindiol production. Evolutionary analysis suggests that the expansion of DcFAD2 genes underpins the abundance of falcarindiol in carrot, independent of the biosynthetic gene cluster previously identified in tomato. This work underscores the complexity of the falcarin biosynthetic network and identifies hub genes essential for falcarindiol biosynthesis in carrot.

Indexed as

Daucus carotaDiynesFatty Acid DesaturasesFatty AlcoholsPlant ProteinsBiosynthetic PathwaysGene Expression Regulation, PlantDiynesfalcarindiolFatty Acid DesaturasesFatty AlcoholsPlant ProteinsDaucus carota Lfalcarindiolfatty acid desaturase 2gene duplicationmetabolic gene clusterpolyacetylenes

Identifiers

PMID40156193
PMCPMC12177475

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.