Evidence map›Paper›PMID 40155175›Full record

ArticleAnalytica chimica acta2025

Nanobody based immunoassay for detection of aquatic virus: Giant salamander iridovirus.

Zhihao Xu, Man Liu, Qiyi He, Wenkai Li, Junkang Pan, El-Sayed A El-Sheikh, Wenzhi Liu, Bruce D Hammock, Dongyang Li

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Zhihao XuCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Hangzhou, 310058, People's Republic of China.
Man LiuYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, People's Republic of China.
Qiyi HeCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Hangzhou, 310058, People's Republic of China.
Wenkai LiCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Hangzhou, 310058, People's Republic of China.
Junkang PanCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Hangzhou, 310058, People's Republic of China.
El-Sayed A El-SheikhFaculty of Agriculture, Zagazig University, Sharkia, Zagazig, 44511, Egypt.
Wenzhi LiuYangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, 430223, People's Republic of China.
Bruce D HammockDepartment of Entomology and Nematology and UCD Comprehensive Cancer Center, University of California Davis, Davis, CA, 95616, United States.
Dongyang LiCollege of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou, 310058, People's Republic of China; Zhejiang Key Laboratory of Intelligent Sensing and Robotics for Agriculture, Hangzhou, 310058, People's Republic of China. Electronic address: dylee@zju.edu.cn.

Funding

Workshop on Environmental Technology Transfer and EntrepreneurshipP42ES004699 · NIEHS · UNIVERSITY OF CALIFORNIA DAVIS · PI YOUNG, THOMAS MICHAEL · 1987 to 2021
$50.1M
Supplement for bioactive lipids as effectors and indicators of the deleterious effects of human healthR35ES030443 · NIEHS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI MORISSEAU, CHRISTOPHE HP · 2019 to 2025
$5.8M
NIEHS NIH HHS P42 ES004699NIEHS NIH HHS R35 ES030443
6 · The paper itself

Abstract

Aquatic viruses cause devastating diseases in aquaculture, severely limiting production and resulting in significant economic losses. The giant salamander iridovirus (GSIV), a member of the genus Ranavirus, is the only virus reported to infect giant salamanders, posing a severe threat to the farming industry. Currently, there are no effective strategies available for its control. In this context, a reliable diagnostic tool for the rapid detection of GSIV is crucial to mitigate its impact. This study developed a nanobody-based immunoassay for the rapid and reliable detection of GSIV. GSIV was cultured and used to immunize an alpaca, A phage library with an original diversity of 1.89 × 10

Indexed as

CaudataIridovirusRanavirusSingle-Domain AntibodiesAnimalsEnzyme-Linked Immunosorbent AssayImmunoassayLimit of DetectionSingle-Domain AntibodiesAquacultureELISAGSIVImmunoassayNanobodypolyHRP

Identifiers

PMID40155175
PMCPMC12118806

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.