ArticleJournal of advanced research2026
Fasting influences the muscle quality of fish during transportation by regulating the balance between energy metabolism and ammonia nitrogen stress.
Article in Journal of advanced research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Recent Advances in the Storage, Transportation, and Quality Control ofFoods (Basel, Switzerland) · 2026Review
- Effect of utilizing a Bacillus sp bacteria consortium for biofilter inoculum in a lab-scale tilapia RAS to enhance production and fish health.Scientific reports · 2026Article
- Effects of Ammonia-Nitrogen-Reducing Biofilm on Stress Responses and Muscle Quality in Crucian Carp During Transportation.Foods (Basel, Switzerland) · 2026Article
- Comprehensive transcriptomic and metabolomic analysis provides insight into transportation stress effects on fish muscle quality.NPJ science of food · 2026Article
- Growth dynamics and compensatory mechanisms in fish under fasting and refeeding regimes.Frontiers in physiology · 2026Review
- Mitigating Effect of Ginger Extract on Survival Rate and Muscle Quality of Crucian Carp (International journal of molecular sciences · 2025Article
- Short-term fasting enhances the resistance of common carp (Veterinary world · 2025Article
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11 authors.
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Abstract
introductionFasting has been widely applied in aquaculture to improve the welfare of fish. However, fasting regulate the interplay of energy metabolism and ammonia nitrogen stress on the muscle quality unclear.
objectivesTo clarify the impact of fasting on the energy metabolism, ammonia nitrogen stress, and muscle quality.
methodsBlunt snout bream (Megalobrama amblycephala) were fasted for different days before transportation. Then changes in energy metabolism, stress response, cell apoptosis, and muscle quality were assessed using UPLC-QTOF-MS untargeted metabolomics along with classical molecular biology techniques.
resultsOur findings revealed that short-term fasting (2-3 d) effectively alleviated ammonia nitrogen stress. The cortisol and superoxide dismutase decreased gradually as the fasting time was extended from 0 to 3 d, with decreased of 28.60 % and 55.39 %. Regarding the energy reserves, a reduction in muscle glycogen, protein, and lipid content was observed after fasting. These changes were attributed to the intensified tricarboxylic acid cycle, amino acid metabolism, and lipid metabolism. Furthermore, fasting enhanced the glycolysis of glycogen, and the gluconeogenesis of glutamic acid and aspartic acid to generate adenosine triphosphate. Notably, short-term fasting alleviated muscle cell apoptosis via down-regulating expression of Caspase 9 and Bax. Moreover, the water holding capacity, shear force, and springiness were better improved after fasting 2 d, reaching 79.88 %, 407.11 g, and 0.78 g, respectively. However, when fasting was extended to 4 d, there was a decrease in energy substances in the fish muscle and an increase in oxidative stress and apoptosis, thereby inducing a decrease in the muscle quality.
conclusionOur data concluded that fasting affects cell apoptosis by regulating the balance between energy metabolism and stress, ultimately impacting muscle quality. For long-distance transportation, a fasting period of 2-3 d is optimal.
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