Evidence map›Paper›PMID 40151055›Full record

ArticleMolecular plant pathology2025

Clade V MLO Genes Are Negative Modulators of Cucumber Defence Response to Meloidogyne incognita.

Xiaoxiao Xie, Jian Ling, Shaoyun Dong, Mingjuan Zhai, Junru Lu, Jianlong Zhao, Xueyong Yang, Xin Dong, Yan Li, Richard G F Visser and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular plant pathology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Xiaoxiao XieState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.ORCID 0009-0009-0897-8410
Jian LingState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Shaoyun DongState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Mingjuan ZhaiState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Junru LuState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Jianlong ZhaoState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Xueyong YangState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Xin DongState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Yan LiState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Richard G F VisserPlant Breeding, Wageningen University & Research, Wageningen, the Netherlands.
Yuling BaiPlant Breeding, Wageningen University & Research, Wageningen, the Netherlands.
Zhenchuan MaoState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.ORCID 0000-0002-1035-7711
Shengping ZhangState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.
Bingyan XieState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, China.

Funding

National Agriculture Science and Technology Major Program NK20220904the National Key R & D Program of China 2023YFD1400400the National Natural Science Foundation of China 31571996the National Natural Science Foundation of China 32372508
6 · The paper itself

Abstract

Cucumber production is seriously constrained by Meloidogyne incognita. Because no resistance resources to the pathogen have been reported, disabling susceptibility genes may represent a novel breeding strategy to introduce resistance against this nematode in cucumber. Here, we studied the clade V MLO genes for their involvement in the interaction between cucumber and M. incognita. Our results showed that Arabidopsis clade V MLO mutants were resistant to M. incognita. Cucumber has three clade V MLO genes, CsaMLO1, CsaMLO8 and CsaMLO11, with upregulated expression upon inoculation with M. incognita. Heterologous overexpression of CsaMLO1, CsaMLO8 and CsaMLO11 in Arabidopsis mutants restored susceptibility to varying degrees. Silencing and knockout of individual clade V MLO genes in cucumber reduced susceptibility to M. incognita. The cucumber CRISPR mutants produced similar fruits as the wild type (WT) did. Although the yields of two single mutants (M11

Indexed as

Cucumis sativusGenes, PlantPlant DiseasesPlant ProteinsTylenchoideaAnimalsArabidopsisDisease ResistanceGene Expression Regulation, PlantMutationPlants, Genetically ModifiedPlant ProteinsCRISPRcucumberMeloidogyne incognitaMLOsusceptibility genesVIGS

Identifiers

PMID40151055
PMCPMC11950635

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.