ArticleAnimals : an open access journal from MDPI2025
Molecular Determination of Sex from Down and Feather in Wild and Reared Monomorphic and Dimorphic Birds at Juvenile Age.
Article in Animals : an open access journal from MDPI, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
2 citing papers in PubMed.
- Tarsus length as a simple and robust candidate for early sex determination in partridges across contrasting growing contexts: a case study in Rock partridge (Veterinary and animal science · 2026Article
- Genetic Sexing of the African Penguin,Ecology and evolution · 2026Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The inability to distinguish males from females in young birds is a major obstacle for pair formation in reintroduction-restocking programs and commercial-amateur breeding. Several techniques are employed to address this problem, but not all of them are suitable for juvenile subjects. Among the various tests applied for sex determination, polymerase chain reaction (PCR) is one of the genetic tools that seems to be most effective (rapid, not invasive and cheaper). In this study, DNA was extracted from down and feathers to make the procedure less stressful for nestlings. The DNA was amplified by PCR, and the amplicon was subjected to the restriction endonucleases procedure when the gender was not clearly identified by PCR alone. One hundred and fifty-three avian species were sexed using this procedure, including 27 for the first time. In all the nestlings and juveniles tested, sex was correctly identified; in fact, all pairs that reached sexual maturity during this study gave offspring.
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