Evidence map›Paper›PMID 40148901›Full record

ArticleBMC oral health2025

Effect of low-level laser on proliferation, angiogenic and dentinogenic differentiation of human dental pulp stem cells.

Fatemeh Rezaei, Shahrzad Shakoori, Mahta Fazlyab, Ehsan Esnaashari, Sohrab Tour Savadkouhi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC oral health, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Trial
  3. Review
  4. Plant Exosome Injection: A New Boost for Postlaser Vascular Repair.The Journal of clinical and aesthetic dermatology · 2026
    Article
  5. Review
  6. Article
  7. Article
  8. Review
  9. Angiogenic regulation of dental pulp stem cells.Frontiers in dental medicine · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Fatemeh RezaeiDepartment of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Shahrzad ShakooriDepartment of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Mahta FazlyabDepartment of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran. Dr.Mfazlyab@gmail.com.ORCID http://orcid.org/0000-0003-0186-2908
Ehsan EsnaashariDepartment of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Sohrab Tour SavadkouhiDepartment of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe aim was to evaluate the effect of single and double doses of low-level laser irradiation on proliferation of human dental pulp stem cells (DPSC) and expression of vascular endothelial growth factor (VEGF) and dentine sialoprotein (DSP).

methodsIn this experimental in vitro study, after confirming the stemness of DPSCs, the cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) for MTT assay and VEGF-ELISA and osteogenic medium for DSP-ELISA. The wells containing DPSCs were divided into three main groups and 9 subgroups (n = 7). In groups with single low-level laser, 660-nm diode laser was irradiated at 100 mW and 3 J/cm

resultsHigher proliferation rate in both single and double low-level laser was reported. The difference was statistically significant for double doses of low-level laser (P = 0.001, P = 0.020 and P = 0.000 for 1, 7 and 14 days, respectively). Also after one, 7 and 14 days, cells in significant increase in DSP (P > 0.05) and VEGF (P > 0.05) was observed that was significantly higher for double doses of low-level laser.

conclusionsLow level laser enhanced the mitochondrial activity and proliferation of DPSCs. Increased production of DSP/VEGF indicates dentinogenic/angiogenic activity. CLINICAL RELEVANCE: Low level laser increases the proliferation of DPSCs, elevates the production of VEGF (which means better angiogenesis in regenerative treatments) and increases the production of DSP (which means better dentinogenesis in vital pulp treatments).

Indexed as

Dental PulpDentinogenesisLow-Level Light TherapyNeovascularization, PhysiologicStem CellsCell DifferentiationCell ProliferationCells, CulturedDentin SialophosphoproteinExtracellular Matrix ProteinsHumansLasers, SemiconductorPhosphoproteinsSialoglycoproteinsVascular Endothelial Growth Factor ADentin SialophosphoproteinExtracellular Matrix ProteinsPhosphoproteinsSialoglycoproteinsVascular Endothelial Growth Factor ADental pulp stem cellsDentine sialoproteinLow-level laser therapyRegenerative endodontic procedureVascular endothelial growth factorVital pulp treatment

Identifiers

PMID40148901
PMCPMC11948823

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.