Green HPLC-Fluorescence detection method for concurrent analysis of Tamsulosin hydrochloride and Tolterodine tartrate in dosage forms and biological fluids.

Sayed M Derayea, Khalid M Badr El-Din, Ahmed S Ahmed, Mohamed Oraby, Mohamed A Abdelshakour

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed

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2 citing papers in PubMed.

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5 authors.

Sayed M DerayeaDepartment of Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt.

Khalid M Badr El-DinDepartment of Analytical Chemistry, Faculty of Pharmacy, Minia University, Minia, 61519, Egypt.

Ahmed S AhmedDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt. ahmed.saad@pharm.sohag.edu.eg.

Mohamed OrabyDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt.

Mohamed A AbdelshakourDepartment of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag University, Sohag, 82524, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel, environmentally friendly HPLC-FL method has been developed to simultaneously quantify tamsulosin hydrochloride (TAM) and tolterodine tartrate (TTD) in pharmaceutical formulations. The method effectively separates the drugs using an ODS column and gradient elution at a flow rate of 1.0 mL/min. Fluorescence detection was used to measure the relative fluorescence intensity (RFI) of the analytes, with excitation and emission wavelengths set at 280 nm and 350 nm, respectively. The retention times for TAM and TTD were determined to be 5.66 and 7.26 min, respectively. The method's accuracy and precision were validated according to ICH guidelines. Linear calibration curves were obtained over the concentration ranges of 0.1-1.5 µg mL

Indexed as

TamsulosinTolterodine TartrateChromatography, High Pressure LiquidFluorescenceGreen Chemistry TechnologyHumansLimit of DetectionReproducibility of ResultsSpectrometry, FluorescenceTabletsTabletsTamsulosinTolterodine TartrateGradient HPLC elutionGreen chemistrySpiked human plasmaSpiked human urineTamsulosinTolterodine

Identifiers

PMID40148346

DOI10.1038/s41598-025-92183-6

PMCPMC11950346

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.