Evidence map›Paper›PMID 40147777›Full record

ArticleJournal of thrombosis and haemostasis : JTH2025

Plasminogen and plasmin induce specialized proresolving mediators and promote efferocytosis via 5-lipoxygenase.

Luiza O Perucci, Fernanda S Carneiro, Josiane C Souza, Laís C Grossi, Jessica A M Souza, Isabella Zaidan, Camila Cardoso, Edvaldo S Lara, Franciel B Felix, Nagyung Baik and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of thrombosis and haemostasis : JTH, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Defective efferocytosis in diabetes: molecular mechanisms and emerging therapeutic strategies.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Luiza O PerucciDepartment of Molecular and Cell Biology, Scripps Research, La Jolla, California, USA; Signaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Fernanda S CarneiroSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Josiane C SouzaSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Laís C GrossiSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Jessica A M SouzaSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Isabella ZaidanSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Camila CardosoSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Edvaldo S LaraSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Franciel B FelixDepartment of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Nagyung BaikDepartment of Molecular and Cell Biology, Scripps Research, La Jolla, California, USA.
Luciana P TavaresDivision of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.
Frederico M SorianiDepartment of Genetics, Ecology and Evolution, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Mauro M TeixeiraDepartment of Biochemistry and Immunology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Robert J ParmerDepartment of Medicine, Veterans Administration San Diego Healthcare System, University of California, San Diego, California, USA.
Lindsey A MilesDepartment of Molecular and Cell Biology, Scripps Research, La Jolla, California, USA.
Lirlândia P SousaSignaling in Inflammation Laboratory, Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Federal University of Minas Gerais, Belo Horizonte, Brazil. Electronic address: lipsousa72@gmail.com.

Funding

Regulation of plasminogen-dependent cell functions by the novel receptor, Plg-RKTR01HL081046 · NHLBI · SCRIPPS RESEARCH INSTITUTE, THE · PI MILES, LINDSEY A · 2007 to 2024
$8.7M
A Novel Plasminogen Receptor Promotes Adipose Function and Metabolic HomeostasisR01HL149511 · NHLBI · SCRIPPS RESEARCH INSTITUTE, THE · PI MILES, LINDSEY A, PARMER, ROBERT J · 2019 to 2022
$3.0M
BLRD VA I01 BX002026NHLBI NIH HHS R01 HL081046NHLBI NIH HHS R01 HL149511
6 · The paper itself

Abstract

backgroundThe plasminogen (Plg)/plasmin (Pla) system has been recognized for its proresolving actions, such as promoting efferocytosis. However, the role of specialized proresolving mediators (SPMs) in these Plg/Pla effects remains unexplored.

objectivesTo investigate whether Plg/Pla promotes SPMs production in macrophages and to elucidate the role of the 5-lipoxygenase (5-LO) pathway in Pla-induced efferocytosis.

methodsBone marrow-derived macrophages (BMDMs) from wild-type (WT) and Plg knockout (KO) mice were treated or not with Plg/Pla, with or without leupeptin (protease inhibitor) and tranexamic acid (lysine analog), for 24 hours. SPMs and leukotriene B4 production and the messenger RNA expression of 5-, 12-, and 15-LO (SPMs biosynthetic enzymes) were assessed in WT-BMDMs. Additionally, 12/15-LO expression was analyzed in pleural macrophages from Pla-injected mice. Efferocytosis of apoptotic neutrophils was examined in Pla-injected WT and 5-LO KO mice and by using the 5-LO inhibitor MK886 in Pla-stimulated BMDMs.

resultsPlg/Pla enhanced the production of lipoxin A

conclusionIn summary, Plg/Pla proresolving actions are associated with SPMs production in macrophages and a 5-LO-dependent pro-efferocytosis mechanism.

Indexed as

Arachidonate 5-LipoxygenaseFibrinolysinMacrophagesPhagocytosisPlasminogenAnimalsApoptosisArachidonate 12-LipoxygenaseArachidonate 15-LipoxygenaseCells, CulturedDocosahexaenoic AcidsEfferocytosisLeukotriene B4MaleMiceMice, Inbred C57BLArachidonate 12-LipoxygenaseArachidonate 15-LipoxygenaseArachidonate 5-LipoxygenaseDocosahexaenoic AcidsFibrinolysinLeukotriene B4Plasminogeninflammation resolutionplasminogen/plasmin systemspecialized proresolving mediators

Identifiers

PMID40147777
PMCPMC12189466

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.