Evidence map›Paper›PMID 40147734›Full record

ArticleNeurochemistry international2025

The C. elegans glutamate transporters GLT-4 and GLT-5 regulate protein expression, behavior, and lifespan.

Grace J Bronstone, Moriah Harton, Maya Muldowney, James Reigle, Adam J Funk, Sinead M O'Donovan, Robert E McCullumsmith, Deborah E Bauer

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Neurochemistry international, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Grace J BronstoneDepartment of Neuroscience, Wellesley College, Science Center, 106 Central Street, Wellesley, MA, 02481, USA. Electronic address: gbronsto@wellesley.edu.
Moriah HartonDepartment of Neuroscience, Wellesley College, Science Center, 106 Central Street, Wellesley, MA, 02481, USA.
Maya MuldowneyDepartment of Neuroscience, Wellesley College, Science Center, 106 Central Street, Wellesley, MA, 02481, USA.
James ReigleDivision of Biomedical Informatics, Cincinnati Children's Hospital Medical Center, 3333 Burnet Avenue, Cincinnati, OH, 45229, USA; Department of Biomedical Informatics, University of Cincinnati College of Medicine, Medical Sciences Building 231 Albert Sabin Way, PO Box 670769, Cincinnati, OH, 45267, USA.
Adam J FunkDepartment of Neuroscience, University of Toledo College of Medicine, 179 Block Health Science Building Mail Stop #1007, 3000 Arlington Avenue, Toledo, OH, 43614, USA.
Sinead M O'DonovanDepartment of Neuroscience, University of Toledo College of Medicine, 179 Block Health Science Building Mail Stop #1007, 3000 Arlington Avenue, Toledo, OH, 43614, USA.
Robert E McCullumsmithDepartment of Neuroscience, University of Toledo College of Medicine, 179 Block Health Science Building Mail Stop #1007, 3000 Arlington Avenue, Toledo, OH, 43614, USA; Neurosciences Institute, ProMedica, 2130 West Central Avenue, Toledo, OH, 43606, USA.
Deborah E BauerDepartment of Neuroscience, Wellesley College, Science Center, 106 Central Street, Wellesley, MA, 02481, USA. Electronic address: dbauer@wellesley.edu.

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
Translational assessment of brain bioenergetic function in schizophreniaR01MH121102 · NIMH · UNIVERSITY OF MARYLAND BALTIMORE · PI MARANO, CHRISTOPHER, MCCULLUMSMITH, ROBERT E · 2020 to 2024
$3.7M
Development of Attenuated Furoxans as Novel Therapies for Alzheimer's DiseaseR01AG057598 · NIA · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI SCHIEFER, ISAAC T · 2018 to 2022
$2.3M
Cell-specific analysis of sub-kinomes in schizophreniaR01MH107487 · NIMH · UNIVERSITY OF TOLEDO HEALTH SCI CAMPUS · PI MCCULLUMSMITH, ROBERT E, MELLER, JAREK · 2016 to 2020
$1.9M
NIA NIH HHS R01 AG057598NIH HHS P40 OD010440NIMH NIH HHS R01 MH107487NIMH NIH HHS R01 MH121102
6 · The paper itself

Abstract

Glutamate transporters are important for regulating extracellular glutamate levels, impacting neural function and metabolic homeostasis. This study explores the behavioral, lifespan, and proteomic profiles in Caenorhabditis elegans strains with either glt-4 or glt-5 null mutations, highlighting contrasting phenotypes. Δglt-4 mutants displayed impaired mechanosensory and chemotactic responses, reduced lifespans, and decreased expression levels of ribosomal proteins and chaperonins involved in protein synthesis and folding. In contrast, Δglt-5 mutants displayed heightened chemorepulsion, extended lifespans, and upregulation of mitochondrial pyruvate carriers and cytoskeletal proteins. Proteomic profiling via mass spectrometry identified 53 differentially expressed proteins in Δglt-4 mutants and 45 in Δglt-5 mutants. Δglt-4 mutants showed disruptions in ribonucleoprotein complex organization and translational processes, including downregulation of glycogen phosphorylase and V-type ATPase subunits, while Δglt-5 mutants revealed altered metabolic protein expression, such as increased levels of mitochondrial pyruvate carriers and decreased levels of fibrillarin and ribosomal proteins. Gene ontology enrichment analysis highlighted differential regulation of protein biosynthesis and metabolic pathways between the strains. Overall, these findings underscore the distinct, tissue-specific roles of GLT-4 and GLT-5 in C. elegans, with broader implications for glutamate regulation and systemic physiology. The results also reinforce the utility of C. elegans as a model for studying glutamate transporters' impact on behavior, longevity, and proteostasis.

Indexed as

Behavior, AnimalCaenorhabditis elegans ProteinsExcitatory Amino Acid Transporter 2LongevityAnimalsCaenorhabditis elegansMutationProteomicsCaenorhabditis elegans ProteinsExcitatory Amino Acid Transporter 2Behavioral phenotypingCaenorhabditis elegansGlutamate transporterLifespan analysisMass spectrometryProteomic profiling

Identifiers

PMID40147734
PMCPMC12053503

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.