Evidence map›Paper›PMID 40147635›Full record

ArticleVirologica Sinica2025

Development of a reporter HBoV1 strain for antiviral drug screening and life cycle studies.

Jielin Tang, Sijie Chen, Yi Zhong, Yijun Deng, Dan Huang, Junjun Liu, Yi Zheng, Jiyuan Xu, Bao Xue, Fan Wang and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virologica Sinica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Jielin TangGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 511436, China. Electronic address: tang_jielin@gzlab.ac.cn.
Sijie ChenGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 511436, China.
Yi ZhongGuangzhou National Laboratory, Guangzhou 510005, China.
Yijun DengGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 511436, China.
Dan HuangGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Junjun LiuGuangzhou National Laboratory, Guangzhou 510005, China.
Yi ZhengState Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Jiyuan XuGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Bao XueGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Fan WangGMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou 511436, China.
Yuan ZhouGuangzhou National Laboratory, Guangzhou 510005, China.
Hanzhong WangState Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, China.
Qi YangGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 511436, China. Electronic address: yang_qi@gzlab.ac.cn.
Xinwen ChenGuangzhou National Laboratory, Guangzhou 510005, China; State Key Laboratory of Respiratory Disease, The First Affiliated Hospital of Guangzhou Medical University, Guangzhou 511436, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human bocavirus 1 (HBoV1; family: Parvoviridae) causes a wide spectrum of respiratory diseases in children and gastroenteritis in adults. A lack of sensitive cell lines and efficient animal models hinders research on HBoV, including the development of anti-HBoV drugs or vaccines. Although the construction of a wild-type HBoV1 infectious clone has been reported, generating HBoV1 infectious clone carrying foreign reporter genes with suitable insertion sites in its genome while retaining replicative ability remains challenging. Here, HBoV1 infectious clones harboring the 11-amino-acid HiBiT tag at five distinct insertion sites were constructed and evaluated. Only the recombinant HBoV1 carrying the HiBiT tag in the N-terminus of the NS1 protein (HBoV1-HiBiT

Indexed as

Antiviral AgentsGenes, ReporterHuman bocavirusAnimalsCell LineDrug Evaluation, PreclinicalHumansIvermectinLuciferasesViral Nonstructural ProteinsVirus ReplicationAntiviral AgentsIvermectinLuciferasesViral Nonstructural ProteinsAntiviral drug evaluationHiBiT tagHuman bocavirus (HBoV)Infectious clone

Identifiers

PMID40147635
PMCPMC12131035

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.