Evidence map›Paper›PMID 40147060›Full record

ArticleStem cell research2025

Generation of an induced pluripotent stem cell line (NCHi023-A) from a 41-year-old male with nemaline myopathy carrying autosomal dominant ACTA1 c.809-10C>A mutation.

Meghan Hanley, Shiqiao Ye, Jingting Zhu, Yang Yu, Hui Lin, Kevin Flanigan, Afrooz Rashnonejad, Ming-Tao Zhao

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PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Meghan HanleyCenter for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH 43215, USA.
Shiqiao YeCenter for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH 43215, USA.
Jingting ZhuCenter for Gene Therapy, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA.
Yang YuCenter for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH 43215, USA.
Hui LinCenter for Gene Therapy, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA.
Kevin FlaniganCenter for Gene Therapy, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
Afrooz RashnonejadCenter for Gene Therapy, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH 43210, USA.
Ming-Tao ZhaoCenter for Cardiovascular Research, The Abigail Wexner Research Institute, Nationwide Children's Hospital, Columbus, OH 43215, USA; The Heart Center, Nationwide Children's Hospital, Columbus, OH 43215, USA; Department of Pediatrics, The Ohio State University College of Medicine, Columbus, OH 43210, USA; Dorothy M Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH 43210, USA. Electronic address: mingtao.zhao@nationwidechildrens.org.

Funding

An integrated human stem cell model for elucidating NOTCH signaling in myocardial-endocardial interactions in cardiac development and diseaseR01HL155282 · NHLBI · RESEARCH INST NATIONWIDE CHILDREN'S HOSP · PI Mingtao Zhao · 2021 to 2026
$3.2M
NHLBI NIH HHS R01 HL155282
6 · The paper itself

Abstract

Nemaline myopathy is a rare genetic condition characterized by weakened muscles due to thread-like rods, called nemaline bodies, in muscle fibers. This condition varies in time of onset and severity. Although there is no cure, therapies and treatments are available to reduce symptoms. This iPSC line, NCHi023-A, was reprogrammed using Sendai virus from skin fibroblasts from a male patient with nemaline myopathy carrying a pathogenic heterozygous ACTA1 mutation. Characterization of this line was successful, with validation of cell identity, normal morphology and karyotype, positive expression of germ layer and pluripotency markers, and negative expression for mycoplasma and transgenes.

Indexed as

ActinsInduced Pluripotent Stem CellsMutationMyopathies, NemalineAdultCell LineFibroblastsHumansMaleACTA1 protein, humanActins

Identifiers

PMID40147060
PMCPMC12038439

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.