Evidence map›Paper›PMID 40146521›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Agrobacterium-Mediated Transformation of Prunus persica (Peach) Immature Cotyledons.

Carolina Toro, Marion Barrera, Blanca Olmedo, Ricardo Vergara, Marisol Muñoz, Felipe Olivares, Humberto Prieto

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Carolina ToroBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile.
Marion BarreraBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile.
Blanca OlmedoBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile.
Ricardo VergaraBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile.
Marisol MuñozBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile.
Felipe OlivaresBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile.
Humberto PrietoBiotechnology Laboratory, La Platina Station, Instituto de Investigaciones Agropecuarias INIA - Chile, La Pintana, Santiago, Chile. hprieto@inia.cl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic transformation in Prunus persica genotypes could add single horticultural traits in existing cultivars without modifying their commercial characteristics. Despite this opportunity, the final setting of transformation protocols in the species endures two major limiting factors preventing the development of new varieties: (a) explants are recalcitrant in regenerating adventitious transformed shoots and (b) have a limited regeneration capability, usually extended to just a few genotypes (i.e., cultivar dependence). This protocol illustrates a procedure that has allowed the establishment of transformation methodologies in several varieties of the species and is presented here for Red Top and Elegant Lady; this methodology could help extend technical baselines for successful transformation procedures in other, potentially more recalcitrant, and newly developed cultivars or genus members.

Indexed as

AgrobacteriumCotyledonPrunus persicaTransformation, GeneticPlants, Genetically ModifiedAgrobacterium-mediated genetic transformation of P. persicaImmature cotyledonsPeach regenerationStable transgene integration

Identifiers

PMID40146521

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.