Evidence map›Paper›PMID 40146512›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Genetic Transformation of Lettuce (Lactuca sativa) Using Agrobacterium tumefaciens.

Damaris Godinez-Vidal, Javier Narváez-Vásquez, Martha L Orozco-Cárdenas

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Damaris Godinez-VidalPlant Transformation Research Center, University of California Riverside, Riverside, CA, USA.
Javier Narváez-VásquezPlant Transformation Research Center, University of California Riverside, Riverside, CA, USA.
Martha L Orozco-CárdenasPlant Transformation Research Center, University of California Riverside, Riverside, CA, USA. mlorozco@ucr.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lettuce (Lactuca sativa L.) plays a pivotal role in global agriculture and food supply chains as a widely cultivated commercial crop. This chapter presents a detailed protocol for transforming lettuce using Agrobacterium tumefaciens. The procedure includes a two-day pre-culture period of cotyledon explants, followed by a 20-min inoculation with Agrobacterium without explant wounding, and a two-day co-culture on the same medium. Afterward, the selection and regeneration phases under kanamycin selection led to the establishment of transgenic plants in the greenhouse. The entire process from the initial explant infection to the greenhouse cultivation of transgenic plants spans roughly 120 days, achieving an average transformation efficiency of 45%. This protocol offers a reliable method for the genetic modification of lettuce, for use in research or crop improvement.

Indexed as

Agrobacterium tumefaciensLactucaTransformation, GeneticCotyledonPlants, Genetically ModifiedAgrobacterium tumefaciensKanamycin resistance geneLettuceNeomycin phosphotransferase II (nptII)Plant transformationTransgenesis

Identifiers

PMID40146512

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.