Evidence map›Paper›PMID 40146509›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Agrobacterium tumefaciens-Mediated Plant Transformation and Gene Editing in Rice.

Mayra Faion-Molina, Marco David Molina-Risco, Marcela Domingues Bellinatti-Della Gracia, Oneida Ibarra, Backki Kim, Endang M Septiningsih, Michael J Thomson

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Mayra Faion-MolinaDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX, USA.
Marco David Molina-RiscoDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX, USA.
Marcela Domingues Bellinatti-Della GraciaDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX, USA.
Oneida IbarraAvance Biosciences Inc., Houston, TX, USA.
Backki KimPlant Genomics and Breeding Institute, Seoul National University, Seoul, South Korea.
Endang M SeptiningsihDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX, USA.
Michael J ThomsonDepartment of Soil and Crop Sciences, Texas A&M University, College Station, TX, USA. m.thomson@tamu.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bottlenecks in plant transformation and regeneration have slowed progress in applying CRISPR/Cas9-based genome editing for crop improvement. Rice (Oryza sativa L.) has highly efficient temperate japonica transformation protocols, along with reasonably efficient indica protocols using immature embryos. However, rapid and efficient protocols are not available for transformation and regeneration in tropical japonica varieties, even though they represent most of the rice production in the USA and South America, along with some regions in Asia. This chapter describes a protocol for CRISPR/Cas9 gene editing using Agrobacterium-mediated transformation for the tropical japonica rice cultivar Presidio leading to knock-out mutations in the phytoene desaturase (PDS) gene.

Indexed as

Agrobacterium tumefaciensGene EditingOryzaTransformation, GeneticCRISPR-Cas SystemsOxidoreductasesPlants, Genetically ModifiedOxidoreductasesphytoene dehydrogenaseAgrobacterium-mediated transformationCRISPR/Cas9Gene editingPhytoene desaturase (PDS)Rice (Oryza sativa L.)Tropical japonica

Identifiers

PMID40146509

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.