Evidence map›Paper›PMID 40146508›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Vacuum-Assisted Transient Agrobacterium tumefaciens-Mediated Transformation for Plant Molecular Analysis.

Triana D'Alençon-Rozzi, Patricio Mora-Figueroa, Christian González-Calquín, Claudia Stange Klein

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Triana D'Alençon-RozziCentro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Patricio Mora-FigueroaCentro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Christian González-CalquínCentro de Biología Molecular Vegetal, Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago, Chile.
Claudia Stange KleinDepartment of Biology, University of Chile, Santiago, Chile. cstange@uchile.cl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Transient plant tissue transformation in Nicotiana tabacum is a valuable technique in molecular biology applications. N. tabacum is highly susceptible to transformation and sustains significant levels of heterologous gene expression. The infiltration process includes an initial phase of T-DNA expression without integration into the genome that can be used for different purposes such as gene expression analysis, quantification of protein accumulation, and metabolite production.To overcome the limitations of conventional infiltration methods, a vacuum infiltration protocol for young N. tabacum plants was developed, allowing simultaneous infiltration of multiple plants. Here, we show the application of the transient agroinfiltration method using the 35S:RUBY plasmid, encoding a biosynthetic pathway for the red-purple betalain pigment to facilitate the visualization of the transformed tissue over time.

Indexed as

Agrobacterium tumefaciensNicotianaTransformation, GeneticDNA, BacterialPlants, Genetically ModifiedPlasmidsVacuumDNA, BacterialT-DNA35S:RUBYAgrobacteriumBetalain productionNicotiana tabacumTransient transformationVacuum infiltration

Identifiers

PMID40146508

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.