ArticleMethods in molecular biology (Clifton, N.J.)2025
Using Agrobacterium tumefaciens to Assemble Multi-step Metabolic Pathways in Nicotiana benthamiana.
Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Chemical modulation of chloroplast de- and redifferentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Chemical modulation of chloroplast de- and re-differentiation reveals a role for the SAL1-PAP retrograde pathway in facilitating plastid transitions.bioRxiv : the preprint server for biology · 2025Article
- Viral vector-based transient expression systems for plant biotechnology research at PUIs.Frontiers in education · 2025Article
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3 authors.
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Abstract
Within the realm of the natural world, plants emerge as prolific producers of diverse bioactive compounds with pharmaceutical, nutritional, and industrial applications. However, many of these compounds are scarce with low concentrations and specific distributions among species, prompting the exploration of methods for producing them in plant biofactories. Typically, pathways comprising several enzymatic steps need to be engineered in plant hosts to produce the desired product of interest from available metabolic precursors. Transient expression systems, specifically agroinfiltration of Nicotiana benthamiana leaves with Agrobacterium tumefaciens, is a potent and cost-effective method for testing synthetic gene combinations. Here, we present a protocol to produce metabolites through a multi-step pathway, exemplifying the assembly of a carotenoid synthesis pathway within the plant cell cytosol. The approach showcases the efficiency and simplicity of agroinfiltration-mediated transient expression systems in reconstructing metabolic pathways, offering a valuable and sustainable alternative to stably transformed lines.
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