ArticleVeterinary medicine and science2025
A Generalized Approach to Genetic Sex Detection of Uniparous Mammalian Species, With High Potential in Foetal Sex Determination Using Circulating Cell-Free DNA of Maternal Plasma.
Article in Veterinary medicine and science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Foetal sex determination of uniparous mammals with long gestation benefits the management of economically important domestic animals as well as captive wild populations, but the various methods currently available are limited to a few species. Based on the hypothesized presence of circulating cell-free foetal DNA (ccff-DNA) in the maternal circulation of relevant species, we aimed to develop a universal genetic assay that has the potential to determine foetal sex in a wide range of species. Highly conserved sequences on the Y chromosome and an autosome were identified and selected to serve as primer annealing sites for a male-specific marker and a reaction control marker, respectively. Primers were designed to amplify short target sequences to a maximum of 300 base pairs (bp) to ensure high sensitivity when applied to degraded foetal DNA. Ultimately, instead of a single universal assay, two sets of primers were designed separately for two groups of the relevant species, resulting in two generalized, highly sensitive duplex PCR assays, each consisting of an SRY (sex-determining region Y) and GAPDH (glyceraldehyde 3-phosphate dehydrogenase) marker. Following preliminary testing and PCR optimization, each assay provided nearly 100% specificity and sensitivity on genomic DNA samples of various species (20 species in total) and maternal plasma samples of an African elephant. Although further validation on maternal plasma samples is necessary, the developed assays have the potential to rapidly promote breeding-related activities of a wide range of species by providing a generalized and therefore inexpensive, rapid and convenient method for foetal sex determination.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.