Evidence map›Paper›PMID 40145774›Full record

ArticleClinical and translational science2025

Robust UPLC-MS/MS Method With Acetonitrile for Precise Intracellular Quantification of Tacrolimus in PBMCs: A Step Toward Clinical Integration.

Napatsanan Tanathitiphuwarat, Asada Leelahavanichkul, Pajaree Chariyavilaskul, Suwasin Udomkarnjananun

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and translational science, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Napatsanan TanathitiphuwaratCenter of Excellence in Clinical Pharmacokinetics and Pharmacogenomics (PKPGx), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
Asada LeelahavanichkulCenter of Excellence on Translational Research in Inflammation and Immunology (CETRII), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.ORCID 0000-0002-5566-6403
Pajaree ChariyavilaskulCenter of Excellence in Clinical Pharmacokinetics and Pharmacogenomics (PKPGx), Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.ORCID 0000-0003-1096-6020
Suwasin UdomkarnjananunCenter of Excellence on Translational Research in Inflammation and Immunology (CETRII), Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.ORCID 0000-0003-4087-9032

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Monitoring whole blood tacrolimus concentrations is standard in clinical practice; however, it may not fully reflect its therapeutic effects, as tacrolimus primarily acts within lymphocytes. While various intracellular quantification methods have been developed, many involve complex procedures such as evaporation, reconstitution, or specialized tools (e.g., magnetic beads, online solid-phase extraction), limiting their accessibility. This study aimed to develop and validate a streamlined, sensitive method for measuring intracellular tacrolimus concentrations using 5×10

Indexed as

Drug MonitoringImmunosuppressive AgentsLeukocytes, MononuclearTacrolimusTandem Mass SpectrometryAcetonitrilesChromatography, High Pressure LiquidHumansLimit of DetectionLiquid Chromatography-Mass SpectrometryReproducibility of ResultsacetonitrileAcetonitrilesImmunosuppressive AgentsTacrolimusintracellular tacrolimusLC–MS/MSperipheral blood mononuclear cellstherapeutic drug monitoring

Identifiers

PMID40145774
PMCPMC11948344

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.