Evidence map›Paper›PMID 40145755›Full record

ArticleApplied and environmental microbiology2025

Growth-coupled continuous directed evolution by MutaT7 enables efficient and automated enzyme engineering.

Yijie Deng, Kai Etheridge, Xinping Ran, Hannah E Maurais, Rahul Sarpeshkar

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied and environmental microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Yijie DengThayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.ORCID 0000-0002-2213-7513
Kai EtheridgeThayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.
Xinping RanDepartment of Biochemical Engineering, Duke University, Durham, North Carolina, USA.
Hannah E MauraisThayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.
Rahul SarpeshkarThayer School of Engineering, Dartmouth College, Hanover, New Hampshire, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traditional directed evolution is limited by labor-intensive iterative steps and low-throughput selection and screening. To address these challenges, we developed a growth-coupled continuous directed evolution (GCCDE) approach, enabling automated and efficient enzyme engineering. By linking enzyme activity to bacterial growth and utilizing the MutaT7 system, GCCDE combines IMPORTANCE: Enzyme engineering aims to develop enzymes with improved or novel traits, but traditional methods are slow and require repetitive manual steps. This study presents a faster, automated protein engineering approach. We utilized an

Indexed as

beta-GalactosidaseDirected Molecular EvolutionEscherichia coliProtein EngineeringPyrococcus furiosusEnzyme StabilityLactoseMutagenesisbeta-GalactosidaseLactosecontinuous evolutionenzyme engineeringgrowth-coupled direction evolutionin vivo mutagenesisMutaT7

Identifiers

PMID40145755
PMCPMC12016552

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.