Evidence map›Paper›PMID 40143682›Full record

ArticleSmall (Weinheim an der Bergstrasse, Germany)2025

Extracellular Bacterial Production of DNA Hydrogels-Toward Engineered Living Materials.

Philipp Gaspers, Christoph Bickmann, Christina Wallner, Daniel Baron Diaz, Dirk Holtmann, Johannes Gescher, Kersten S Rabe, Christof M Niemeyer

Abstract read
In one paragraph

Article in Small (Weinheim an der Bergstrasse, Germany), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Philipp GaspersInstitute for Biological Interfaces 1 (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.ORCID 0009-0008-5148-1496
Christoph BickmannInstitute for Biological Interfaces 1 (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
Christina WallnerInstitute of Technical Microbiology, University of Technology Hamburg (TUHH), Kasernenstraße 12, 21073, Hamburg, Germany.
Daniel Baron DiazInstitute of Process Engineering in Life Sciences 2 - Electrobiotechnology, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 4, 76131, Karlsruhe, Germany.
Dirk HoltmannInstitute of Process Engineering in Life Sciences 2 - Electrobiotechnology, Karlsruhe Institute of Technology (KIT), Fritz-Haber-Weg 4, 76131, Karlsruhe, Germany.
Johannes GescherInstitute of Technical Microbiology, University of Technology Hamburg (TUHH), Kasernenstraße 12, 21073, Hamburg, Germany.ORCID 0000-0002-1625-8810
Kersten S RabeInstitute for Biological Interfaces 1 (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.ORCID 0000-0001-7909-8191
Christof M NiemeyerInstitute for Biological Interfaces 1 (IBG 1), Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.ORCID 0000-0002-8837-081X

Funding

Bundesministerium für Bildung und Forschung 13XP5152BHelmholtz Association 43.33.11
6 · The paper itself

Abstract

Engineered Living Materials (ELMs) combine synthetic biology with artificial materials to create biohybrid living systems capable of replicating, self-repairing, and responding to external stimuli. Due to their self-optimization abilities, these systems hold great potential for biotechnological applications. This study is a first step toward ELMs based on DNA hydrogels, focusing on the production of biohybrid materials using the exoelectrogenic bacterium Shewanella oneidensis. To equip the bacterium with the functionality needed for building DNA hydrogels, inducible cell surface anchors are developed, which can bind exogenous polymerase via the SpyCatcher/SpyTag (SC/ST) technology. The process parameters for in situ production of DNA hydrogels are established, enabling the development of these materials in the context of living bacteria for the first time. Using an extracellular nuclease-deficient S. oneidensis strain, stable biohybrid biofilms are generated directly on the surface of bioelectrochemical systems, showing the current generation. Given the high programmability and functionalization potential of DNA hydrogels, it is believed that this study represents a significant step toward establishing dynamic biohybrid material systems that exhibit both conductivity and metabolic activity.

Indexed as

DNAHydrogelsShewanellaBiofilmsDNAHydrogelsBESbiofilmsDNA materialsengineered living materialsS. oneidensis

Identifiers

PMID40143682
PMCPMC12067159

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.