ArticleEuropean endodontic journal2025
Comparative Evaluation of the Effect of EDTA, Chitosan, Etidronic Acid, and Silver Citrate on the Mineral Content of Root Canal Dentin Using Energy Dispersive X-ray Spectroscopy: An In-Vitro Study.
Article in European endodontic journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Efficacy of Etidronic Acid for Smear Layer Removal: A Systematic Review of In Vitro Studies.Journal of functional biomaterials · 2026Review
- Evaluation of EDTA and silver nanoparticle based root canal irrigants on smear layer elimination and dentine surface roughness.Bioinformation · 2026Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveSodium hypochlorite is a common irrigant in endodontics, used to eliminate microorganisms and dissolve pulpal tissue. However, adjunctive chelating agents, while aiding in smear layer removal, can reduce dentinal wall microhardness, affecting dentin permeability, solubility, and the sealing ability of root canal sealers. This study aims to evaluate the impact of newer chelating agents-Silver Citrate, Chitosan, HEBP, and EDTA-on the mineral composition of root canal dentin using energy-dispersive X-ray Spectroscopy (EDXS).
methodsThe root canals of 120 freshly extracted human mandibular premolars with single straight canals and intact, mature apices were prepared following standard endodontic procedures using Rotary Pro Taper nickel-titanium files. Final irrigation was conducted with 5 mL of the respective chelating solution. Changes in the mineral composition of the root canal dentin were analyzed using EDXS. Descriptive statistics were presented as means and standard deviations. The impact of chelating solutions on the mineral content of root canal dentin at the cervical, middle, and apical thirds was compared between groups using One-Way ANOVA followed by a post hoc Bonferroni test.
resultsThe highest mean concentrations of minerals such as Mg, Ca, and P at both the coronal and apical levels (p<0.05), along with the Ca/P ratio in the coronal third, were observed with distilled water, followed by HEBP, Chitosan, EDTA, and Silver Citrate, which showed the lowest values. In the middle third, the concentrations of Ca and P, as well as the Ca/P ratio in the apical and middle thirds, followed the sequence: Distilled Water>EDTA>HEBP>Chitosan>Silver Citrate. The difference in the levels of all minerals was statistically significant (p<0.05) at the coronal, middle and apical third levels.
conclusionAmong the tested solutions, 9% HEBP caused the least alteration in the mineral content of root canal dentin when compared to 0.2% Chitosan, 17% EDTA, and Silver Citrate. This suggests that HEBP may be a preferable choice in endodontic procedures where minimal alteration of dentin mineral content is desired. (EEJ-2024-08-124).
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