Evidence map›Paper›PMID 40143287›Full record

ArticleViruses2025

Establishment of a Visual Gene Chip Method for the Simultaneous Detection of Seven Waterfowl Virus Pathogens.

Linjie Yan, Yafen Song, Tianshu Zhai, Qian Qiu, Jia Wang, Jinming Liu, Daiyue Lv, Xiaojie Huang, Huabin Cao, Chenghuai Yang and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Update on Reovirus Infections in Waterfowls.Animals : an open access journal from MDPI · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Linjie YanChina Institute of Veterinary Drug Control, Beijing 100081, China.
Yafen SongChina Institute of Veterinary Drug Control, Beijing 100081, China.
Tianshu ZhaiChina Institute of Veterinary Drug Control, Beijing 100081, China.
Qian QiuJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Jia WangChina Institute of Veterinary Drug Control, Beijing 100081, China.
Jinming LiuChina Institute of Veterinary Drug Control, Beijing 100081, China.
Daiyue LvChina Institute of Veterinary Drug Control, Beijing 100081, China.
Xiaojie HuangChina Institute of Veterinary Drug Control, Beijing 100081, China.
Huabin CaoJiangxi Provincial Key Laboratory for Animal Health, Institute of Animal Population Health, College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China.
Chenghuai YangChina Institute of Veterinary Drug Control, Beijing 100081, China.
Yaqing MaoChina Institute of Veterinary Drug Control, Beijing 100081, China.

Funding

China Institute of Veterinary Drug Control 2023115757329
6 · The paper itself

Abstract

Goose parvovirus (GPV), duck enteritis virus (DEV), Muscovy duck parvovirus (MDPV), duck hepatitis A virus type 1 (DHAV-1), duck hepatitis A virus type 3 (DHAV-3), duck Tembusu virus (DTMUV), and novel duck reovirus (NDRV) are significant pathogens that spread extensively among waterfowl populations, causing economic losses for the waterfowl industry. In order to detect seven pathogens simultaneously, a visual gene chip for the detection of multiple waterfowl disease pathogens was developed in this study. The gene chip was capable of specifically amplifying GPV, DEV, MDPV, DHAV-1, -DHAV-3, DTMUV, and NDRV. The sensitivity results showed that the lowest detection limit of the gene chip was 1 copy/μL for single and mixed samples. The reproducibility and stability tests demonstrated that the gene chip developed in this experiment exhibited not only excellent reproducibility but also remarkable stability, remaining functional for a minimum of 180 days. Compared to qPCR methods, the results showed that the sensitivity of the gene chip was slightly better than that of the qPCR method in detecting both single and mixed pathogens of the seven viruses. In this study, a total of 210 clinical samples were detected by the gene chip and qPCR, respectively, and the results of the two methods had a concordance rate of 98.1~100%, with a kappa value of 0.952, indicating that the consistency of the two detection methods was good.

Indexed as

Bird DiseasesOligonucleotide Array Sequence AnalysisPoultry DiseasesVirus DiseasesVirusesAnimalsDucksReproducibility of ResultsSensitivity and Specificitygene chipmolecular diagnosticspathogenvirusesvisualisationwaterfowl

Identifiers

PMID40143287
PMCPMC11945778

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.