Evidence map›Paper›PMID 40143285›Full record

ArticleViruses2025

AliMarko: A Pipeline for Virus Identification Using an Expert-Guided Approach.

Nikolay Popov, Ignat Sonets, Anastasia Evdokimova, Maria Molchanova, Vera Panova, Elena Korneenko, Alexander Manolov, Elena Ilina

Abstract read
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nikolay PopovResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.ORCID 0009-0002-6450-8594
Ignat SonetsResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.ORCID 0009-0009-7266-6657
Anastasia EvdokimovaIndependent Researcher, 393410 Tambov, Russia.
Maria MolchanovaResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.ORCID 0009-0009-8034-5474
Vera PanovaResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.
Elena KorneenkoResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.
Alexander ManolovResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.
Elena IlinaResearch Institute for Systems Biology and Medicine (RISBM) of Rospotrebnadzor, 117246 Moscow, Russia.ORCID 0000-0003-0130-5079

Funding

Russian State 122030900069-4
6 · The paper itself

Abstract

Viruses are ubiquitous across all kingdoms of cellular life, posing a significant threat to human health, and analyzing viral communities is challenging due to their genetic diversity and lack of a single, universally conserved marker gene. To address this challenge, we developed the AliMarko pipeline, a tool designed to streamline virus identification in metagenomic data. Our pipeline uses a dual approach, combining mapping reads with reference genomes and a de novo assembly-based approach involving an HMM-based homology search and phylogenetic analysis, to enable comprehensive detection of viral sequences, including low-coverage and divergent sequences. We applied our pipeline to total RNA sequencing of bat feces and identified a range of viruses, quickly validating viral sequences and assessing their phylogenetic relationships. We hope that the AliMarko pipeline will be a useful resource for the scientific community, facilitating the interpretation of viral communities and advancing our understanding of viral diversity and its impact on human health.

Indexed as

Computational BiologyMetagenomicsVirusesAnimalsChiropteraFecesGenetic VariationGenome, ViralHumansPhylogenyautomated pipelinephylogenetic analysisviromevirus discovery

Identifiers

PMID40143285
PMCPMC11946232

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.