Evidence map›Paper›PMID 40143274›Full record

ArticleViruses2025

Lineage B Genotype III of Dengue Virus Serotype 3 (DENV-3III_B) Is Responsible for Dengue Outbreak in Dire Dawa City, Ethiopia, 2023.

Abebe Aseffa Negeri, Dawit Hailu Alemayehu, Saro Abdella Abrahim, Tsigereda Kifle Wolde, Gutema Bulti Tura, Alemnesh Hailemariam Bedasso, Danile Tsega Geretsion, Ebise Abose Djirata, Eyilachew Zenebe Awule, Diana Rojas-Gallardo and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Abebe Aseffa NegeriEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.
Dawit Hailu AlemayehuArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.ORCID 0000-0002-4118-8260
Saro Abdella AbrahimEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.
Tsigereda Kifle WoldeEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.
Gutema Bulti TuraEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.ORCID 0009-0008-7492-9046
Alemnesh Hailemariam BedassoEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.ORCID 0000-0001-7762-8208
Danile Tsega GeretsionEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.ORCID 0009-0004-6396-8024
Ebise Abose DjirataEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.
Eyilachew Zenebe AwuleDire Dawa City Administration Health Bureau, Dire Dawa P.O. Box 1377, Ethiopia.
Diana Rojas-GallardoPopulation Biology, Ecology and Evolution Graduate Program, Emory University, Atlanta, GA 30322, USA.ORCID 0000-0002-7902-2231
Asefa Konde KorkisoEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.
Kalkidan MelakuArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.
Raffael JosephArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.ORCID 0009-0009-8915-552X
Abaysew AyeleArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.
Mesfin Mengesha TsegayeArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.ORCID 0000-0002-7756-9110
Anne PiantadosiDepartment of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA.ORCID 0000-0002-5942-1534
Getachew TolleraEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.
Alemseged AbdissaArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.
Mesay Hailu DangisoEthiopian Public Health Institute, Addis Ababa P.O. Box 1242, Ethiopia.ORCID 0000-0003-1245-8839
Adane MihretArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.
Andargachew MuluArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.ORCID 0000-0003-2862-0175
Tesfaye GelanewArmauer Hansen Research Institute, Addis Ababa P.O. Box 1005, Ethiopia.ORCID 0000-0002-0696-3786

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The eastern parts of Ethiopia, including Dire Dawa City, have experienced annual dengue fever (DF) outbreaks since 2013, leading to significant healthcare and economic impacts. However, comprehensive evidence on the specific dengue virus (DENV) serotypes and genotypes involved remains limited. During the 2023 DF outbreak, the National Arbovirus Laboratory received seventy serum samples from suspected DF patients. Positive samples underwent sequencing of the CprM region of the DENV genome, and the obtained sequences were analyzed phylogenetically. Among the patients, 32 (45.7%) displayed early warning signs of severe dengue, and 13 were hospitalized, most showing symptoms indicative of severe dengue. Out of 67 adequate samples, 44 (65.6%) tested positive for DENV RNA by RT-PCR, and 17 successfully underwent CprM sequencing. All sequenced samples were identified as DENV-3, genotype III, major lineage B (DENV-3III_B), with two distinct minor lineages (DENV-3III_B.2 and DENV-3III_B.3). Phylogenetic analysis showed that these lineages were closely related to sequences from the Afar region, suggesting interconnected outbreaks with multiple co-circulating lineages. This study identifies DENV-3III_B as the cause of the 2023 DF outbreak in Dire Dawa City and highlights the need for enhanced viral genomic surveillance in Africa.

Indexed as

DengueDengue VirusDisease OutbreaksGenotypeAdolescentAdultChildChild, PreschoolEthiopiaFemaleGenome, ViralHumansMaleMiddle AgedPhylogenyRNA, ViralRNA, ViralCprMDENVDENV-3/GIIIDFDire Dawa CityEthiopiagenotypeserotype

Identifiers

PMID40143274
PMCPMC11945396

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.