Evidence map›Paper›PMID 40142527›Full record

ArticleMicroorganisms2025

Interpreting Microbial Species-Area Relationships: Effects of Sequence Data Processing Algorithms and Fitting Models.

Fu-Liang Qi, Wei Deng, Yi-Ting Cheng, Xiao-Yan Yang, Na Li, Wen Xiao

Abstract read
In one paragraph

Article in Microorganisms, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Fu-Liang QiInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China.
Wei DengInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China.ORCID 0000-0003-4918-7550
Yi-Ting ChengInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China.
Xiao-Yan YangInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China.ORCID 0000-0002-9304-308X
Na LiInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China.ORCID 0000-0002-2697-2268
Wen XiaoInstitute of Eastern-Himalaya Biodiversity Research, Dali University, Dali 671003, China.ORCID 0000-0002-4951-7524

Funding

National Natural Science Foundation of China 32371557
6 · The paper itself

Abstract

In the study of Species-Area Relationships (SARs) in microorganisms, outcome discrepancies primarily stem from divergent high-throughput sequencing data processing algorithms and their combinations with different fitting models. This paper investigates the impacts and underlying causes of using diverse sequence data processing algorithms in microbial SAR studies, as well as compatibility issues that arise between different algorithms and fitting models. The findings indicate that the balancing strategies employed by different algorithms can result in variations in the calculations of alpha and beta diversity, thereby influencing the SARs of microorganisms. Crucially, incompatibilities exist between algorithms and models, with no consistently optimal combination identified. Based on these insights, we recommend prioritizing the use of the DADA2 algorithm in conjunction with a power model, which demonstrates greater compatibility. This study serves as a comprehensive comparison and reference for fundamental methods in microbial SAR research. Future microbial SAR studies should carefully select the most appropriate algorithms and models based on specific research objectives and data structures.

Indexed as

high-throughput sequencingmicrobial diversity patternsmodel fittingspecies–area relationship

Identifiers

PMID40142527
PMCPMC11944932

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.