Single-Port Fluorescence Immunoassay for Concurrent Quantification of Live and Dead Bacteria: A Strategy Based on Extracellular Nucleases and DNase I.

Yuhan Wang, Han Dong, Hang Yu, Shaofeng Yuan, Hideya Kawasaki, Yahui Guo, Weirong Yao

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

7 authors.

Yuhan WangState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Han DongState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Hang YuState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.ORCID 0000-0001-7927-0473

Shaofeng YuanState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Hideya KawasakiFaculty of Chemistry, Materials and Bioengineering, Kansai University, Suita 564-8680, Japan.ORCID 0000-0003-2713-2057

Yahui GuoState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.ORCID 0000-0002-6512-355X

Weirong YaoState Key Laboratory of Food Science and Resources, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Funding

the Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases (Jiangsu Provincial Center for Disease Control and Prevention) PM202403the Science and Technology Planning Project of Jiangsu Market Supervision and Administration Bureau KJ2024086

6 · The paper itself

Abstract

Bacteria are the primary culprits of global foodborne diseases, making bacterial detection one of the most critical aspects of food safety. The quantification of viable and dead bacteria is typically achieved through distinct methodologies, such as culture-based methods and molecular biological techniques. These approaches often have non-overlapping requirements in terms of sample pre-treatment and detection equipment. However, in this presented work, bacterial extracellular nucleases and DNase I were utilized to achieve the simultaneous quantification of both live and dead bacteria in a single well of a microplate. The detection limits of the method for live and dead bacteria are estimated to be 7.13 × 10

Indexed as

BacteriaDeoxyribonuclease IDeoxyribonucleasesFood MicrobiologyLimit of DetectionMicrobial ViabilityDeoxyribonuclease IDeoxyribonucleasesaptamersextracellular nucleasefoodborne pathogensimmunoassayviable bacteria detection

Identifiers

PMID40142149

DOI10.3390/molecules30061374

PMCPMC11944870

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.