Smart Probes for Ultrasensitive and Highly Selective Sensing of Homocysteine over Cysteine Based on Multi-Cooperative Effects by Using Gold Nanoparticles.

Manman Sun, Peihao Zhang, Zeze Xie, Pengcheng Zhang, Zhendong Li, Zhiguang Yang, Hongyu Chen

Abstract read

In one paragraph

Article in Molecules (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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Authors and funding

7 authors.

Manman SunCollege of Physics and Telecommunication Engineering, Zhoukou Normal University, Zhoukou 466001, China.

Peihao ZhangCollege of Physics and Telecommunication Engineering, Zhoukou Normal University, Zhoukou 466001, China.

Zeze XieHenan Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China.

Pengcheng ZhangHenan Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China.

Zhendong LiHenan Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China.ORCID 0000-0003-2403-9244

Zhiguang YangHenan Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China.

Hongyu ChenHenan Key Laboratory of Rare Earth Functional Materials, College of Chemistry and Chemical Engineering, Zhoukou Normal University, Zhoukou 466001, China.

Funding

Doctoral Scientific Research Foundation of Zhoukou Normal University ZKNUC2022012Key Research Projects of Henan Higher Education Institutions 25A150038Key Scientific and Technological Project of Henan Province 212102210289National Natural Science Foundation of China 12305076Natural Science Foundation of Henan Province 232300420396

6 · The paper itself

Abstract

Homocysteine (Hcy) is a biothiol that plays a vital role in many physiological processes and is involved in a variety of diseases. However, it is significantly difficult to discriminate Hcy from cysteine (Cys) due to their similar chemical structures (only one methylene difference) and reactivity. In this study, a novel nanosensor was proposed to discriminate Hcy from Cys with multi-cooperative effects by using gold nanoparticles (AuNPs). The discrimination effect for Hcy originates from the interaction difference of the hydrogen bonding, steric hindrance, and carbon chain length in Hcy and Cys with AuNPs. Under the best conditions, this nanosensor has two unique advantages. Firstly, the sensor exhibits high sensitivity with detection limits of 0.1 μM through naked-eye determination and 0.008 μM through UV-vis spectroscopy analysis. Secondly, the sensor showed superior selectivity for Hcy over the other 16 natural amino acids (biothiol-containing Cys and glutathione (GSH)), and it is the first time to clearly distinguish Hcy from Cys (the Cys concentration is 40 times higher than Hcy). Furthermore, the system was further employed to detect Hcy in human serum, and the result was in agreement with that tested by clinicians via enzymatic assays, with acceptable recovery.

Indexed as

Biosensing TechniquesCysteineGoldHomocysteineMetal NanoparticlesHumansLimit of DetectionCysteineGoldHomocysteinecolorimetric sensinggold nanoparticleshigh selectivityhigh sensitivityhomocysteinemulti-cooperative effects

Identifiers

PMID40142083

DOI10.3390/molecules30061309

PMCPMC11946112

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