Evidence map›Paper›PMID 40140835›Full record

ArticleBMC biology2025

Ectopic expression of Slc1a2 in the prefrontal cortex of sleep-deprived male mice counteracts the glutamate/GABA-glutamine dysfunction.

Fengying Zhang, Yao Li, Li Jiang, Yingbin Wang, Yonghong Tang, Bo Ouyang, Guojun Song, Xuan Li, Xiajie Quan, Min Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Fengying Zhang *Department of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, Hunan Province, 421001, China.
Yao Li *Department of Physiology, Jinzhou Medical University, Jinzhou, 121000, China.
Li Jiang *Department of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, Hunan Province, 421001, China.
Yingbin WangDepartment of Neurosurgery, Central Hospital Affiliated to Shenyang Medical College, Shenyang, 110024, China.
Yonghong TangDepartment of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, Hunan Province, 421001, China.
Bo OuyangDepartment of Sleep Medical Centre, the Affiliated Nanhua Hospital,Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Guojun SongDepartment of Radiology, The Affiliated Nanhua Hospital, University of South China, Hengyang, Hunan, 421001, China.
Xuan LiDepartment of Docimasiology, Hengyang Medical School, The Affiliated Nanhua Hospital, University of South China, Hengyang, 421002, Hunan, China.
Xiajie QuanDepartment of Sleep Medical Centre, the Affiliated Nanhua Hospital,Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Min LiDepartment of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, Hunan Province, 421001, China.
Hongying WangDepartment of Respiratory and Critical Care Medicine, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, Hengyang, Hunan, 421001, China.
Zhao PanDepartment of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, Hunan Province, 421001, China.
Keyan ChenLaboratory Animal Science of China Medical University, No.77 Puhe Road, Shenyang, Liaoning, 110122, China. kychen@cmu.edu.cn.
Ping ZhangDepartment of Neurology, the Affiliated Nanhua Hospital, Hengyang Medical School, University of South China, No. 336, Dongfeng South Road, Zhuhui District, Hengyang, Hunan Province, 421001, China. zhangp-usc@foxmail.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe prefrontal cortex (PFC) plays a pronounced role in cognitive and emotional functions, which may be compromised by dismal sleep quality. This study intended to clarify the impact of Slc1a2 ectopic expression in the PFC on sleep deprivation (SD)-induced disturbances in the glutamate (Glu)/GABA-glutamine cycle and the role of astrocyte (AC)-neuron (Neu) communication.

methodsSingle-cell RNA sequencing was adopted to illuminate cell-specific changes in the brainstem, cortex, and hypothalamus of mice under NS, SD, and post-SD conditions. Cell communication analysis was applied to study interactions between ACs and Neus, which altered after the SD. Slc1a2 was ectopically expressed in the PFC and subjected to SD, followed by electrophysiological, immunofluorescence staining, and [

resultsSD caused notable changes in cellular distribution and downregulation of metabolic and synaptic genes in affected brain regions. Cell communication studies highlighted a reduction in AC-Neu interactions, with corresponding metabolic disruptions in the Glu/GABA-glutamine cycle as depicted by [

conclusionsSlc1a2 ectopic expression in the PFC negates SD-induced GABA dysfunction through vital AC-Neu communication. This study sheds light on the mechanisms through which SD affects neural function and suggesting potential treatments for sleep-related disorders.

Indexed as

gamma-Aminobutyric AcidGlutamic AcidGlutaminePrefrontal CortexSleep DeprivationAnimalsExcitatory Amino Acid Transporter 2MaleMiceMice, Inbred C57BLNeuronsExcitatory Amino Acid Transporter 2gamma-Aminobutyric AcidGlutamic AcidGlutamineSlc1a2 protein, mouseAstrocyte-neuron interactionGlutamate/GABA-glutamine cyclePrefrontal cortexSlc1a2Sleep deprivation

Identifiers

PMID40140835
PMCPMC11948699

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.