ArticleScientific reports2025
Identification, characterization, and function analysis of the VIT family in Phaeodactylum tricornutum.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Funding
Abstract
Iron is an essential microelement for all living organisms. The vacuolar iron transporters (VIT) gene family is found in various species, including yeast, fungi, protozoa, and plants, where it plays a crucial role in sequestration, homeostasis, and tolerance of the heavy metals, particularly iron and manganese. However, the presence and function of VIT genes in marine phytoplankton have not been previously reported. The study aims to identify the VIT family within the marine diatom Phaeodactylum tricornutum and to analyze the function of these genes. We conducted a comprehensive analysis of the VIT genes in P. tricornutum genome, examining their phylogenetic relationship, physicochemical properties, gene structures, conserved motifs, domains, expression profile, and cis-acting elements using in silico methods. Function analysis were performed through complementation experiments and the expression of eGFP fusion protein in yeast. Four members of the VIT family were identified in P. tricornutum. All belonging to the VTL (VIT like) group in phylogenetic tree and containing a VIT1 domain. These genes are distributed across chromosomes 2, 4, and 13, with tandem duplication of the PtVTL1 and PtVTL2 contributed to the expansion of this gene family. Expression profile showed that the PtVTL3 is induced to express highly under light condition, others are induced to express highly under dark. PtVTL2 is highly induced to express at low Fe condition, and PtVTL3 is highly induced to express at high Fe condition. Analysis of cis-acting regulatory elements indicated that these genes are primarily involved in responses to environmental stress and phytohormones. Heterologous expression of PtVTL3 successfully rescued the iron-sensitive phenotype in yeast mutant △ccc1. The expression of eGFP-PtVTL3 fusion protein in yeast demonstrated that PtVTL3 is located to the tonoplast. These findings suggest that PtVTL3 function to transport Fe
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.